Calcein tetraethyl ester

• CAT Number: I013799
• CAS Number: 1170856-93-5
• Molecular Formula: C₃₈H₄₂N₂O₁₃
• Molecular Weight: 734.75
• Purity: ≥95%
• Target: Dye Reagents
• Solubility: 10 mM in DMSO
• IUPAC Name: ethyl 2-[[5'-[[bis(2-ethoxy-2-oxoethyl)amino]methyl]-3',6'-dihydroxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-4'-yl]methyl-(2-ethoxy-2-oxoethyl)amino]acetate
• InChI: InChI=1S/C38H42N2O13/c1-5-48-31(43)19-39(20-32(44)49-6-2)17-24-29(41)15-13-27-35(24)52-36-25(18-40(21-33(45)50-7-3)22-34(46)51-8-4)30(42)16-14-28(36)38(27)26-12-10-9-11-23(26)37(47)53-38/h9-16,41-42H,5-8,17-22H2,1-4H3
• InChIKey: AWKMLDYACNCHOZ-UHFFFAOYSA-N
• SMILES: CCOC(=O)CN(CC1=C(C=CC2=C1OC3=C(C24C5=CC=CC=C5C(=O)O4)C=CC(=C3CN(CC(=O)OCC)CC(=O)OCC)O)O)CC(=O)OCC

Calcein Tetraethyl Ester (CAT: I013799) is a cell-permeable fluorescent dye widely used in cell viability and cytotoxicity assays. Once inside live cells, intracellular esterases hydrolyze it into calcein, a highly fluorescent, membrane-impermeable compound that emits bright green fluorescence. This property makes it ideal for tracking cell health, proliferation, and membrane integrity in cell biology and drug screening research. Calcein tetraethyl ester is particularly useful in live/dead assays, calcium flux studies, and multidrug resistance research, providing a sensitive and non-toxic method for monitoring intracellular processes and evaluating the effects of experimental treatments.