CellTracker Blue CMF2HC Dye

• CAT Number: I043071
• CAS Number: 215868-45-4
• Molecular Formula: C10H5ClF2O3
• Molecular Weight: 246.59
• Purity: ≥95%
• Synonyms: 4-(chloromethyl)-6,8-difluoro-7-hydroxychromen-2-one
• InChI: InChI=1S/C10H5ClF2O3/c11-3-4-1-7(14)16-10-5(4)2-6(12)9(15)8(10)13/h1-2,15H,3H2
• InChIKey: QDLNIWVLVXWCPH-UHFFFAOYSA-N
• SMILES: C1=C(C2=CC(=C(C(=C2OC1=O)F)O)F)CCl

CellTracker Blue CMF2HC Dye is a blue dye, can be used in two-channel nuclei acid sequencing, with blue and purple excitation light (450-460 nm/400-405nm or 415-450 nm/480-525nm). CellTracker Blue CMF2HC Dye can be used to rapid determination of antibiotic sensitivity of microorganisms[1][2].
CellTracker Blue CMF2HC Dye is a blue dye that can be excitable by a blue light source having a wavelength of about 450-460 nm, is used as the first or the second detectable label described herein[1].
Blue/Violet Two-Channel Sequencing Methods[1]:
1.Contacting a primer polynucleotide/taiget polynucleotide complex with a mixture comprising one or more of a first type of nucleotide, a second type of nucleotide, a third type of nucleotide, and a fourth type of nucleotide, wherein the primer polynucleotide is complementary to at least a portion ofthe single stranded target polynucleotide;
2.Incorporating one type of nucleotide from the mixture to the primer polynucleotide to produce an extended primer polynucleotide (i.e., an extended primer polynucleotide/target polynucleotide complex);
3.Performing a first imaging event using a first excitation light source and collecting a first emission signal from the extended primer polynucleotide/target polynucleotide complex with a first emission filer;
4.Performing a second imaging event using a second excitation light source and collecting a second emission signal from the extended primer polynucleotide/taiget polynucleotide complex with a second emission filter;
Note[1]:
a. one of the first excitation light source and the second excitation light source has a wavelength of about 350 nm to about 410 nm, and the other one of the first excitation light source and the second excitation light source has a wavelength of about 450 nm to about 460 nm;
b. one of the first emission filter and the second emission filter has a detection wavelength of about 415 nm to about 450 nm, and the other one of the first emission filter and the second emission filter has a detection wavelength of about 480 nm to about 525 nm.

Reference

[1]. Wu Xiaolin, et al. Methods and compositions for nucleic acid sequencing: US, US20220195518[P]. 2022-06-23.

[2]. Super Michael, et al. Rapid antibiotic susceptibility testing: US, US20150064703[p]. 2015-03-05.