For research use only. Not for therapeutic Use.
CellTracker Red CMTPX is a cell-permeable fluorescent dye that can be used as a cell tracer for monitoring cell movement and location (Ex/Em=586/614 nm)[1].
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Fixation and staining of self-assembled spheroids[1]:
1. Resuspended cells are stained with CellTracker Red CMTPX and allowed to self-assemble into spheroids before aggregation.
2. Cell aggregates are fixed in 4% paraformaldehyde/PBS for 30 min with gentle inversion, then washing cells in PBS for 3 times and stained with 100 ng/mL Hoechst dye overnight prior to imaging.
| Catalog Number | I042052 |
| Synonyms | 4-[[4-(chloromethyl)benzoyl]amino]-2-(6,7,7,9,17,19,19,20-octamethyl-2-oxonia-6,20-diazapentacyclo[12.8.0.03,12.05,10.016,21]docosa-1,3,5(10),8,11,13,15,17,21-nonaen-13-yl)benzoate;5-[[4-(chloromethyl)benzoyl]amino]-2-(6,7,7,9,17,19,19,20-octamethyl-2-oxonia-6,20-diazapentacyclo[12.8.0.03,12.05,10.016,21]docosa-1,3,5(10),8,11,13,15,17,21-nonaen-13-yl)benzoate |
| Molecular Formula | C42H40ClN3O4 |
| Purity | ≥95% |
| InChI | InChI=1S/2C42H40ClN3O4/c1-23-20-41(3,4)45(7)34-18-36-32(16-29(23)34)38(33-17-30-24(2)21-42(5,6)46(8)35(30)19-37(33)50-36)31-15-27(13-14-28(31)40(48)49)44-39(47)26-11-9-25(22-43)10-12-26;1-23-20-41(3,4)45(7)34-18-36-32(16-29(23)34)38(33-17-30-24(2)21-42(5,6)46(8)35(30)19-37(33)50-36)28-14-13-27(15-31(28)40(48)49)44-39(47)26-11-9-25(22-43)10-12-26/h2*9-21H,22H2,1-8H3,(H-,44,47,48,49) |
| InChIKey | NUAZOHBLTNHBQC-UHFFFAOYSA-N |
| SMILES | CC1=CC(N(C2=CC3=[O+]C4=CC5=C(C=C4C(=C3C=C12)C6=C(C=C(C=C6)NC(=O)C7=CC=C(C=C7)CCl)C(=O)[O-])C(=CC(N5C)(C)C)C)C)(C)C.CC1=CC(N(C2=CC3=[O+]C4=CC5=C(C=C4C(=C3C=C12)C6=C(C=CC(=C6)NC(=O)C7=CC=C(C=C7)CCl)C(=O)[O-])C(=CC(N5C)(C)C)C)C)(C)C |
| Reference | [1]. Van Pel DM, et al. Modelling glioma invasion using 3D bioprinting and scaffold-free 3D culture. J Cell Commun Signal. 2018 Dec;12(4):723-730. |
