Cellufluor

• CAT Number: I024359
• CAS Number: 4193-55-9
• Molecular Formula: C40H42N12Na2O10S2
• Molecular Weight: 960.95
• Purity: 98%
• Synonyms: Kayaphor FB; Paper White BP; Phorwite AR; Tinopal 5BMS
• Solubility: Soluble in DMSO
• Appearance: Solid powder
• Storage: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
• IUPAC Name: disodium;5-[[4-anilino-6-[bis(2-hydroxyethyl)amino]-1,3,5-triazin-2-yl]amino]-2-[(E)-2-[4-[[4-anilino-6-[bis(2-hydroxyethyl)amino]-1,3,5-triazin-2-yl]amino]-2-sulfonatophenyl]ethenyl]benzenesulfonate
• InChI: InChI=1S/C40H44N12O10S2.2Na/c53-21-17-51(18-22-54)39-47-35(41-29-7-3-1-4-8-29)45-37(49-39)43-31-15-13-27(33(25-31)63(57,58)59)11-12-28-14-16-32(26-34(28)64(60,61)62)44-38-46-36(42-30-9-5-2-6-10-30)48-40(50-38)52(19-23-55)20-24-56;;/h1-16,25-26,53-56H,17-24H2,(H,57,58,59)(H,60,61,62)(H2,41,43,45,47,49)(H2,42,44,46,48,50);;/q;2*+1/p-2/b12-11+
• InChIKey: YJHDFAAFYNRKQE-YHPRVSEPSA-L
• SMILES: O=S(C1=CC(NC2=NC(N(CCO)CCO)=NC(NC3=CC=CC=C3)=N2)=CC=C1/C=C/C4=CC=C(NC5=NC(N(CCO)CCO)=NC(NC6=CC=CC=C6)=N5)C=C4S(=O)([O-])=O)([O-])=O.[Na+].[Na+]

Cellufluor (Cat.No:I024359) is a fluorescent dye used to label and visualize cell wall carbohydrates in various organisms, including bacteria, fungi, and plants. It specifically binds to β-1,3-glucans, which are essential components of fungal and bacterial cell walls. Cellufluor is commonly used in research to study cell wall biosynthesis, morphogenesis, and dynamics. It can also be used for diagnostic purposes to identify fungal infections in clinical specimens.

Reference

1: Milligan T. Cellufluor staining of bronchoalveolar lavage samples. J Clin Microbiol. 1992 Mar;30(3):754. PubMed PMID: 1372623; PubMed Central PMCID: PMC265151.
2: Baselski VS, Robison MK, Pifer LW, Woods DR. Rapid detection of Pneumocystis carinii in bronchoalveolar lavage samples by using Cellufluor staining. J Clin Microbiol. 1990 Feb;28(2):393-4. PubMed PMID: 1690218; PubMed Central PMCID: PMC269620.
3: Sutphin JE, Robinson NM, Wilhelmus KR, Osato MS. Improved detection of oculomycoses using induced fluorescence with cellufluor. Ophthalmology. 1986 Mar;93(3):416-7. PubMed PMID: 3703513.
4: Perry JL, Miller GR. Quality control slide for potassium hydroxide and cellufluor fungal preparations. J Clin Microbiol. 1989 Jun;27(6):1411-2. PubMed PMID: 2754012; PubMed Central PMCID: PMC267574.
5: Stewart A, Deacon JW. Vital fluorochromes as tracers for fungal growth studies. Biotech Histochem. 1995 Mar;70(2):57-65. PubMed PMID: 7578589.
6: Rüchel R, Margraf S. Rapid microscopical diagnosis of deep-seated mycoses following maceration of fresh specimens and staining with optical brighteners. Mycoses. 1993 Jul-Aug;36(7-8):239-42. PubMed PMID: 7509452.
7: Gottlieb S, Altboum Z, Savage DC, Segal E. Adhesion of Candida albicans to epithelial cells effect of polyoxin D. Mycopathologia. 1991 Sep;115(3):197-205. PubMed PMID: 1749403.
8: Monheit JG, Brown G, Kott MM, Schmidt WA, Moore DG. Calcofluor white detection of fungi in cytopathology. Am J Clin Pathol. 1986 Feb;85(2):222-5. PubMed PMID: 2418677.
9: Maudgal PC. Acanthamoeba keratitis: report of three cases. Bull Soc Belge Ophtalmol. 1989;231:135-48. PubMed PMID: 2488446.
10: Dall L, Herndon B. Quantitative assay of glycocalyx produced by viridans group streptococci that cause endocarditis. J Clin Microbiol. 1989 Sep;27(9):2039-41. PubMed PMID: 2476465; PubMed Central PMCID: PMC267733.
11: Belliveau DJ, Garbary DJ, McLachlan JL. Effects of fluorescent brighteners on growth and morphology of the red alga Antithamnion kylinii. Stain Technol. 1990;65(6):303-11. PubMed PMID: 2080526.
12: Vandenbosch KA, Noel KD, Kaneko Y, Newcomb EH. Nodule initiation elicited by noninfective mutants of Rhizobium phaseoli. J Bacteriol. 1985 Jun;162(3):950-9. PubMed PMID: 3997785; PubMed Central PMCID: PMC215868.
13: Chander J, Chakrabarti A, Sharma A, Saini JS, Panigarhi D. Evaluation of Calcofluor staining in the diagnosis of fungal corneal ulcer. Mycoses. 1993 Jul-Aug;36(7-8):243-5. PubMed PMID: 7509453.
14: Hector RF, Braun PC. A 96-well epifluorescence assay for rapid assessment of compounds inhibitory to Candida spp. J Clin Microbiol. 1986 Oct;24(4):620-4. PubMed PMID: 2429985; PubMed Central PMCID: PMC268983.
15: Easson DD Jr, Sinskey AJ, Peoples OP. Isolation of Zoogloea ramigera I-16-M exopolysaccharide biosynthetic genes and evidence for instability within this region. J Bacteriol. 1987 Oct;169(10):4518-24. PubMed PMID: 2820929; PubMed Central PMCID: PMC213816.
16: Shea EM, Gibeaut DM, Carpita NC. Structural analysis of the cell walls regenerated by carrot protoplasts. Planta. 1989 Oct;179(3):293-308. doi: 10.1007/BF00391074. PubMed PMID: 24201658.
17: Luna VA, Stewart BK, Bergeron DL, Clausen CR, Plorde JJ, Fritsche TR. Use of the fluorochrome calcofluor white in the screening of stool specimens for spores of microsporidia. Am J Clin Pathol. 1995 May;103(5):656-9. PubMed PMID: 7537939.