CH3OCO-D-CHA-Gly-Arg-pNA acetate

• CAT Number: I040914
• CAS Number: 80895-10-9
• Molecular Formula: C27H42N8O9
• Molecular Weight: 622.67
• Purity: ≥95%
• Synonyms: acetic acid;methyl N-[(2R)-3-cyclohexyl-1-[[2-[[(2S)-5-(diaminomethylideneamino)-1-(4-nitroanilino)-1-oxopentan-2-yl]amino]-2-oxoethyl]amino]-1-oxopropan-2-yl]carbamate
• InChI: InChI=1S/C25H38N8O7.C2H4O2/c1-40-25(37)32-20(14-16-6-3-2-4-7-16)22(35)29-15-21(34)31-19(8-5-13-28-24(26)27)23(36)30-17-9-11-18(12-10-17)33(38)39;1-2(3)4/h9-12,16,19-20H,2-8,13-15H2,1H3,(H,29,35)(H,30,36)(H,31,34)(H,32,37)(H4,26,27,28);1H3,(H,3,4)/t19-,20+;/m0./s1
• InChIKey: QXWRKXQCMBOLJR-CMXBXVFLSA-N
• SMILES: CC(=O)O.COC(=O)NC(CC1CCCCC1)C(=O)NCC(=O)NC(CCCN=C(N)N)C(=O)NC2=CC=C(C=C2)[N+](=O)[O-]

CH3OCO-D-CHA-Gly-Arg-pNA acetate(CAT: I040914) is a peptide substrate designed for enzyme activity assays, particularly to study proteases or enzymes involved in hydrolyzing peptide bonds. The structure consists of a protective methyl ester group (CH3OCO), a D-CHA (D-chlorohydroxyacetyl) moiety, and a peptide sequence with glycine (Gly), arginine (Arg), and a p-nitroaniline (pNA) group. The pNA group, when cleaved by specific enzymes, produces a yellow color that can be quantified spectrophotometrically, making it a useful tool for enzyme kinetics studies. This compound is relevant for enzyme research, pharmaceutical development, and material chemistry applications, specifically for protease inhibitor screening or enzymatic assays.