CHAPS

• CAT Number: I004800
• CAS Number: 75621-03-3
• Molecular Formula: C32H58N2O7S
• Molecular Weight: 614.88
• Purity: ≥95%
• Synonyms: 3-[dimethyl-[3-[[(4R)-4-[(3R,5S,7R,8R,9S,10S,12S,13R,14S,17R)-3,7,12-trihydroxy-10,13-dimethyl-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthren-17-yl]pentanoyl]amino]propyl]azaniumyl]propane-1-sulfonate
• Target: HDAC
• Solubility: H2O: ≥ 46 mg/mL
• Storage: Store at -20°C
• InChI: InChI=1S/C32H58N2O7S/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41)/t21-,22+,23-,24-,25+,26+,27-,28+,30+,31+,32-/m1/s1
• InChIKey: UMCMPZBLKLEWAF-BCTGSCMUSA-N
• SMILES: CC(CCC(=O)NCCC[N+](C)(C)CCCS(=O)(=O)[O-])C1CCC2C1(C(CC3C2C(CC4C3(CCC(C4)O)C)O)O)C

CHAPS (CAT: I004800) is a zwitterionic detergent commonly used in biochemical and biophysical research for solubilizing and stabilizing membrane proteins. It is a mild and non-denaturing detergent that helps maintain the native structure and function of membrane proteins during purification and analysis. CHAPS interacts with the hydrophobic regions of membrane proteins, disrupting lipid-protein interactions and facilitating their solubilization in aqueous solutions. Due to its zwitterionic nature, CHAPS is compatible with various experimental techniques such as gel electrophoresis, chromatography, and spectroscopy. Its ability to effectively solubilize membrane proteins makes CHAPS a valuable tool in studying their structure, function, and interactions in biological systems.

Reference

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5. Krofli? A, Sarac B, Be?ter-Roga? M. Thermodynamic characterization of 3-[(3-cholamidopropyl)-dimethylammonium]-1-propanesulfonate (CHAPS) micellization using isothermal titration calorimetry: temperature, salt, and pH dependence. Langmuir. 2012 Jul 17;28(28):10363-71.