CHIR-99021 monohydrochloride

• CAT Number: I002334
• CAS Number: 1797989-42-4
• Molecular Formula: C22H19Cl3N8
• Molecular Weight: 501.80
• Purity: ≥95%
• Target: Stem Cell/Wnt
• Solubility: 10 mM in DMSO
• Storage: -20°C
• IUPAC Name: 6-[2-[[4-(2,4-dichlorophenyl)-5-(5-methyl-1H-imidazol-2-yl)pyrimidin-2-yl]amino]ethylamino]pyridine-3-carbonitrile;hydrochloride
• InChI: InChI=1S/C22H18Cl2N8.ClH/c1-13-10-29-21(31-13)17-12-30-22(32-20(17)16-4-3-15(23)8-18(16)24)27-7-6-26-19-5-2-14(9-25)11-28-19;/h2-5,8,10-12H,6-7H2,1H3,(H,26,28)(H,29,31)(H,27,30,32);1H
• InChIKey: SCQDMKUZHIGAIB-UHFFFAOYSA-N
• SMILES: CC1=CN=C(N1)C2=CN=C(N=C2C3=C(C=C(C=C3)Cl)Cl)NCCNC4=NC=C(C=C4)C#N.Cl

CHIR-99021 monohydrochloride（Cat No.:I002334）is the hydrochloride salt form of CHIR-99021, a potent and selective inhibitor of glycogen synthase kinase-3 (GSK-3), specifically targeting both GSK-3α and GSK-3β isoforms. It effectively activates the Wnt/β-catenin signaling pathway, which is essential for regulating cell proliferation, differentiation, and stem cell renewal. This compound is widely used in stem cell research to promote the self-renewal and maintenance of pluripotency in embryonic stem cells. Additionally, CHIR-99021 monohydrochloride has potential therapeutic applications in treating neurodegenerative diseases, diabetes, and cancer by modulating GSK-3-related pathways.

Reference

[1]. Ring DB et al. Selective glycogen synthase kinase 3 inhibitors potentiate insulin activation of glucose transport and utilization in vitro and in vivo. Diabetes. 2003 Mar;52(3):588-95.

[2]. Weikel KA et al. Knockdown of GSK3β increases basal autophagy and AMPK signalling in nutrient-laden human aortic endothelial cells. Biosci Rep. 2016 Sep 16;36(5).

[3]. Ai Z et al. CHIR99021 enhances Klf4 Expression through β-Catenin Signaling and miR-7a Regulation in J1 Mouse Embryonic Stem Cells. PLoS One. 2016 Mar 3;11(3)

[4]. Preston GC et al. Single cell tuning of Myc expression by antigen receptor signal strength and interleukin-2 in T lymphocytes. EMBO J. 2015 Aug 4;34(15):2008-24.

[5]. Preston GC et al. Single cell tuning of Myc expression by antigen receptor signal strength and interleukin-2 in T lymphocytes. EMBO J. 2015 Aug 4;34(15):2008-24.