CHIR 99021 trihydrochloride

• CAT Number: I002310
• CAS Number: 1782235-14-6
• Molecular Formula: C₂₂H₁₈Cl₂N₈. 3HCl
• Molecular Weight: 574.72
• Purity: ≥95%
• Target: Stem Cell/Wnt
• Solubility: DMSO: ≥ 32 mg/mL
• Storage: 3 years -20C powder
• IUPAC Name: 6-[2-[[4-(2,4-dichlorophenyl)-5-(5-methyl-1H-imidazol-2-yl)pyrimidin-2-yl]amino]ethylamino]pyridine-3-carbonitrile;trihydrochloride
• InChI: InChI=1S/C22H18Cl2N8.3ClH/c1-13-10-29-21(31-13)17-12-30-22(32-20(17)16-4-3-15(23)8-18(16)24)27-7-6-26-19-5-2-14(9-25)11-28-19;;;/h2-5,8,10-12H,6-7H2,1H3,(H,26,28)(H,29,31)(H,27,30,32);3*1H
• InChIKey: DSFVSCNMMZRCIA-UHFFFAOYSA-N
• SMILES: CC1=CN=C(N1)C2=CN=C(N=C2C3=C(C=C(C=C3)Cl)Cl)NCCNC4=NC=C(C=C4)C#N.Cl.Cl.Cl

CHIR 99021 trihydrochloride（Cat No.:I002310）is a potent and selective inhibitor of glycogen synthase kinase-3 (GSK-3), targeting both GSK-3α and GSK-3β isoforms. By inhibiting GSK-3, CHIR 99021 enhances Wnt/β-catenin signaling, making it a valuable tool for stem cell research and regenerative medicine. It is widely used to promote self-renewal in pluripotent stem cells and to induce differentiation in specific cellular lineages. Additionally, CHIR 99021 is explored for its potential in treating neurodegenerative diseases, diabetes, and cancer, where GSK-3 plays a key regulatory role in disease progression.

Reference

[1]. Ring DB et al. Selective glycogen synthase kinase 3 inhibitors potentiate insulin activation of glucose transport and utilization in vitro and in vivo. Diabetes. 2003 Mar;52(3):588-95.
[2]. Weikel KA et al. Knockdown of GSK3β increases basal autophagy and AMPK signalling in nutrient-laden human aortic endothelial cells. Biosci Rep. 2016 Sep 16;36(5).
[3]. Ai Z et al. CHIR99021 enhances Klf4 Expression through β-Catenin Signaling and miR-7a Regulation in J1 Mouse Embryonic Stem Cells. PLoS One. 2016 Mar 3;11(3)
[4]. Preston GC et al. Single cell tuning of Myc expression by antigen receptor signal strength and interleukin-2 in T lymphocytes. EMBO J. 2015 Aug 4;34(15):2008-24.
[5]. Preston GC et al. Single cell tuning of Myc expression by antigen receptor signal strength and interleukin-2 in T lymphocytes. EMBO J. 2015 Aug 4;34(15):2008-24.