For research use only. Not for therapeutic Use.
CM-H2DCFDA is a derivative of H2DCFDA (HY-D0940). CM-H2DCFDA can be used to determine cellular oxidant levels (Ex/Em: 495/530 nm). CM-H2DCFDA is light-sensitive[1].
Guidelines[2] (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
ROS Measurement:
1. Incubate the cells according to your normal protocol.
2. Treat cells with 10 μM CM-H2DCFDA for 30 min at 37°C in darkness.
3. Wash the excess probe.
4. Analyze sample on a flow cytometer, fluorescence microscopy, or fluorescence microplate reader.
| Catalog Number | I042559 |
| CAS Number | 850013-49-9 |
| Synonyms | acetyl 2-(3,6-diacetyloxy-2,7-dichloro-9H-xanthen-9-yl)benzoate;chloroethane |
| Molecular Formula | C27H19Cl3O8 |
| Purity | ≥95% |
| InChI | InChI=1S/C26H18Cl2O8.C2H5Cl/c1-12(29)33-23-10-21-17(8-19(23)27)25(15-6-4-5-7-16(15)26(32)35-14(3)31)18-9-20(28)24(34-13(2)30)11-22(18)36-21;1-2-3/h4-11,25H,1-3H3;2H2,1H3 |
| InChIKey | JOMNIEDHXZVHEU-UHFFFAOYSA-N |
| SMILES | CCCl.CC(=O)OC1=C(C=C2C(C3=CC(=C(C=C3OC2=C1)OC(=O)C)Cl)C4=CC=CC=C4C(=O)OC(=O)C)Cl |
| Reference | [1]. Oparka M, et al. Quantifying ROS levels using CM-H2DCFDA and HyPer. Methods. 2016 Oct 15;109:3-11. [2]. Kolarova H, et al. Production of reactive oxygen species after photodynamic therapy by porphyrin sensitizers. Gen Physiol Biophys. 2008 Jun;27(2):101-5. |
