For research use only. Not for therapeutic Use.
Coelenterazine is a luciferin, a light-<wbr></wbr>emitting biomolecule that serves as a substrate for luciferases or as a constituent of photoproteins. The oxidation of coelenterazine to coelenteramide is accompanied by emission of blue light (emission maximum = 460-<wbr></wbr>470 nm). Luciferase-<wbr></wbr>mediated oxidation of coelenterazine or a derivative is used as an energy donor, typically to a form of green fluorescent protein (GFP), in bioluminescent resonance energy transfer (BRET) studies. Coelenterazine 400a is a bisdeoxy derivative of coelenterazine that produces an emission peak near 400 nm following conversion by <em>Renilla</em> luciferase (Rluc). It is used in BRET2 protocols, whereas coelenterazine h, which has a 475 nm Rluc emission peak, is used in BRET1 protocols. The emission peak of coelenterazine 400a is commonly paired with class 1 and 3 GFPs, including GFP2 and GFP10. BRET2 assays are commonly used in evaluating protein-<wbr></wbr>protein interactions, including those involved in G protein-<wbr></wbr>coupled receptor signaling.
| Catalog Number | R065216 |
| CAS Number | 70217-82-2 |
| Synonyms | DeepBlueC |
| Molecular Formula | C26H21N3O |
| Purity | ≥95% |
| Storage | -20°C |
| IUPAC Name | 2,8-dibenzyl-6-phenyl-7H-imidazo[1,2-a]pyrazin-3-one |
| InChI | InChI=1S/C26H21N3O/c30-26-23(17-20-12-6-2-7-13-20)28-25-22(16-19-10-4-1-5-11-19)27-24(18-29(25)26)21-14-8-3-9-15-21/h1-15,18,27H,16-17H2 |
| InChIKey | MWOYPGFRXPPBOX-UHFFFAOYSA-N |
| SMILES | C1=CC=C(C=C1)CC2=C3N=C(C(=O)N3C=C(N2)C4=CC=CC=C4)CC5=CC=CC=C5 |
| Reference | 1.Sabnis, R.W. Handbook of biological dyes and stains: Synthesis and industrial applications. (2010). |
