Coppersensor 1

• CAT Number: I045276
• CAS Number: 874748-20-6
• Molecular Formula: C30H50BF2N3S4
• Molecular Weight: 629.81
• Purity: ≥95%

Coppersensor-1 (CS1) is a membrane-permeable fluorescent dye. Coppersensor-1 has a picomolar affinity for Cu+ with high selectivity over competing cellular metalions. Coppersensor-1 as a probe, can selective and sensitive detection of copper(I) ions (Cu+) in biological samples, including live cells. Coppersensor-1 can be used for the research of imaging of severe diseases such as cancer, cardiovascular disorders and neurogenerative diseases[1].
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
A. Preparation of reagent stock solutions for imaging:
1. Prepare a 1 mM stock solution of CS1 (MW 630 g/moL) in DMSO by dissolving 0.63 mg of solid CS1 per milliliter of DMSO solvent.
2. Prepare a 10 mM stock solution of CuCl2 (MW 134 g/moL) by dissolving 1.34 mg of solid CuCl2 per milliliter of Millipore water solvent.
B. CS1 labeling of live cells:
1. Imaging experiments with cells will require 1-2 d with cells in culture. Incubate the cells according to your normal protocol.
2. One day before imaging, cells are passaged and plated on 18-mm glass coverslips coated with poly-L-lysine (50 μg/mL). Adherent cells for imaging are grown to 50-80% confluency.
3. Remove cells from incubator, and transfer one coverslip into a 35-mm Petri dish containing 3 mL PBS buffer.
4. To the Petri dish, add 15 μL of a 1 mM CS1 stock solution to give a final dye concentration of 5 μM. Mix thoroughly.
(Higher concentrations of dye may result in high levels of background fluorescence)
5. Incubate for 5-20 min in the dark at 25 or 37℃.
C. Imaging the CS1-labeled cells:
1. Imageing in standard confocal microscope with 543 nm excitation.
(CS1 can be imaged using any type of fluorescence microscope, including epifluorescence, confocal and multiphoton)

Reference

[1]. Evan W Miller, et al. Preparation and use of Coppersensor-1, a synthetic fluorophore for live-cell copper imaging. Nat Protoc. 2006;1(2):824-7.
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