For research use only. Not for therapeutic Use.
CUG (3-Carboxyumbelliferyl-β-D-galactopyranoside)(CAT: I013027) is a sensitive fluorogenic substrate commonly used to detect β-galactosidase enzyme activity. Upon enzymatic cleavage by β-galactosidase, CUG releases 3-carboxyumbelliferone, a highly fluorescent product that emits measurable fluorescence, enabling real-time monitoring of enzyme kinetics. This substrate is widely applied in biochemical assays, cell biology, and microbiology for studying gene expression, especially in systems where β-galactosidase serves as a reporter gene. Its high sensitivity and specificity make CUG a valuable tool in the Biochemical Reagents field for applications in enzyme activity analysis, drug screening, and diagnostic assay development.
Catalog Number | I013027 |
CAS Number | 64664-99-9 |
Molecular Formula | C₁₆H₁₆O₁₀ |
Purity | ≥95% |
Target | Dye Reagents |
Solubility | DMSO |
IUPAC Name | 2-oxo-7-[(2S,3R,4S,5R,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxychromene-3-carboxylic acid |
InChI | InChI=1S/C16H16O10/c17-5-10-11(18)12(19)13(20)16(26-10)24-7-2-1-6-3-8(14(21)22)15(23)25-9(6)4-7/h1-4,10-13,16-20H,5H2,(H,21,22)/t10-,11+,12+,13-,16-/m1/s1 |
InChIKey | HGMXXIAQZWTZLR-WUGLTUCPSA-N |
SMILES | C1=CC2=C(C=C1OC3C(C(C(C(O3)CO)O)O)O)OC(=O)C(=C2)C(=O)O |