Cyanine3 hydrazide

• CAT Number: I025332
• Molecular Formula: C30H40N4O2+
• Molecular Weight: 472.67
• Purity: 98%
• Solubility: Soluble in DMSO
• Appearance: Solid powder
• Storage: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
• SMILES: CC(C1=CC=CC=C1N/2C)(C)C2=C/C=C/C3=[N+](CCCCCC(N[NH3+])=O)C4=CC=CC=C4C3(C)C.[2Cl-]

Cyanine3 hydrazide (Cat No.: I025332) is a fluorescent dye commonly used in molecular biology and biochemistry for labeling and imaging applications. It features a cyanine dye backbone, which emits fluorescence when excited by specific wavelengths of light, making it useful for tracking molecules in biological systems. The hydrazide group allows for conjugation with other molecules, such as proteins or peptides, enabling targeted labeling. Cyanine3 hydrazide is often employed in fluorescence microscopy, flow cytometry, and various assays to study cellular processes, gene expression, and biomolecular interactions.

Reference

1. Zhang, J.; Chetnani, B.; Cormack, E.D.; Alonso, D.; Liu, W.; Mondragon, A.; Fei, J. Specific structural elements of the T-box riboswitch drive the two-step binding of the tRNA ligand. eLife, 2018, 7, e39518. doi: 10.7554/eLife.39518
2. Cui, Z.; Wu, Y.; Mureev, S.; Alexandrov, K. Oligonucleotide-mediated tRNA sequestration enables one-pot sense codon reassignment in vitro. Nucleic Acids Research, 2018, 46(12), 6387–6400. doi: 10.1093/nar/gky365
3. Porciani, D.; Cardwell, L.N.; Tawiah, K.d.; Alam, K.K.; Lange, M.J.; Daniels, M.A.; Burke, D.H. Modular cell-internalizing aptamer nanostructure enables targeted delivery of large functional RNAs in cancer cell lines. Nature Communications, 2018, 9(1), 2283. doi: 10.1038/s41467-018-04691-x
4. Poudyal, R.R.; Nguyen, P.D.M.; Lokugamage, M.P.; Callaway, M.K.; Gavette, J.V.; Krishnamurthy, R.; Burke, D.H. Nucleobase modification by an RNA enzyme. Nucleic Acids Research, 2017, 45(3), 1345–1354. doi: 10.1093/nar/gkw1199
5.Boone, C.; Grove, R.; Adamcova, D.; Braga, C.; Adamec, J. Revealing oxidative damage to enzymes of carbohydrate metabolism in yeast: An integration of 2D DIGE, quantitative proteomics and bioinformatics. Proteomics, 2016, 16(13), 1889–1903. doi: 10.1002/pmic.201500546