For research use only. Not for therapeutic Use.
D-3, a phosphorpeptide, is an efficient, simple, and specific iPSC-eliminating agent[1].
D-3 prevents residual iPSC-induced teratoma formation in a mouse tumorigenicity assay[1].
D-3 induces obvious loss of viability in 201B7 cells, with half maximal inhibitory concentration value of 192.3 ± 57.4 μM[1].
D-3 induces an increase in the amount of activated elF2a, p38, and p44/42 MAPK, which
are activated in response to cellular stress. D-3 also increases the number of Annexin-V and SYTOX-positive cells, indicating apoptotic and dead cells after D-3 treatment[1].
D-3 has little influence on various non-iPSCs, including hepatocytes and neurons[1].
| Catalog Number | I015115 |
| CAS Number | 1967815-98-0 |
| Synonyms | (2R)-2-[[(2R)-2-[[(2R)-2-[[(2R)-2-[(2-naphthalen-2-ylacetyl)amino]-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-3-(4-phosphonooxyphenyl)propanoic acid |
| Molecular Formula | C48H47N4O10P |
| Purity | ≥95% |
| InChI | InChI=1S/C48H47N4O10P/c53-44(31-36-20-23-37-18-10-11-19-38(37)26-36)49-40(27-32-12-4-1-5-13-32)45(54)50-41(28-33-14-6-2-7-15-33)46(55)51-42(29-34-16-8-3-9-17-34)47(56)52-43(48(57)58)30-35-21-24-39(25-22-35)62-63(59,60)61/h1-26,40-43H,27-31H2,(H,49,53)(H,50,54)(H,51,55)(H,52,56)(H,57,58)(H2,59,60,61)/t40-,41-,42-,43-/m1/s1 |
| InChIKey | VSVONENCEIBGNL-MRWFHJSOSA-N |
| SMILES | C1=CC=C(C=C1)CC(C(=O)NC(CC2=CC=CC=C2)C(=O)NC(CC3=CC=CC=C3)C(=O)NC(CC4=CC=C(C=C4)OP(=O)(O)O)C(=O)O)NC(=O)CC5=CC6=CC=CC=C6C=C5 |
| Reference | [1]. Yi Kuang, et al. Efficient, Selective Removal of Human Pluripotent Stem Cells via Ecto-Alkaline Phosphatase-Mediated Aggregation of Synthetic Peptides. Cell Chem Biol. 2017 Jun 22;24(6):685-694.e4. |
