For research use only. Not for therapeutic Use.
DAF-2DA (5,6-Diaminofluorescein diacetat) is most widely probe for NO measurement.
Nitric oxide is a free radical signal molecule. Various methods are available for measurement of NO. Out of all methods, fluorescent probes to localize NO is very widely used method. Diaminofl uorescein in diacetate form (DAF-2DA) is most widely probe for NO measurement. This method is based on application of 4,5-diaminofl uorescein diacetate (DAF-2DA) which is actively diffused into cells, once taken up by cells cytoplasmic esterases cleave the acetate groups to generate 4,5-diaminofl uorescein; DAF-2. The generated DAF-2 can readily react with N2O3, which is an oxidation product of NO to generate the highly fluorescent DAF-2T (triazolofl uorescein).
| Catalog Number | R067376 |
| CAS Number | 205391-02-2 |
| Synonyms | (6′-acetyloxy-5,6-diamino-3-oxospiro[2-benzofuran-1,9′-xanthene]-3′-yl) acetate |
| Molecular Formula | C24H18N2O7 |
| Purity | ≥95% |
| InChI | InChI=1S/C24H18N2O7/c1-11(27)30-13-3-5-16-21(7-13)32-22-8-14(31-12(2)28)4-6-17(22)24(16)18-10-20(26)19(25)9-15(18)23(29)33-24/h3-10H,25-26H2,1-2H3 |
| InChIKey | PTSUYDXEEKDBQU-UHFFFAOYSA-N |
| SMILES | CC(=O)OC1=CC2=C(C=C1)C3(C4=C(O2)C=C(C=C4)OC(=O)C)C5=CC(=C(C=C5C(=O)O3)N)N |
| Reference | [1]. Wany A, et al. Localization of Nitric Oxide in Wheat Roots by DAF Fluorescence. Methods Mol Biol. 2016;1424:39-47. |
