DC_AC50

• CAT Number: I011671
• CAS Number: 497061-48-0
• Molecular Formula: C17H12BrF2N3OS
• Molecular Weight: 424.26
• Purity: ≥95%
• Synonyms: 3-amino-N-(2-bromo-4,6-difluorophenyl)-6,7-dihydro-5H-cyclopenta[b]thieno[3,2-e]pyridine-2-carboxamide
• Target: Apoptosis
• Solubility: Soluble in DMSO
• Storage: Store at -20 ℃
• InChI: InChI=1S/C17H12BrF2N3OS/c18-10-5-8(19)6-11(20)14(10)23-16(24)15-13(21)9-4-7-2-1-3-12(7)22-17(9)25-15/h4-6H,1-3,21H2,(H,23,24)
• InChIKey: DFNOJNBNTVQPCA-UHFFFAOYSA-N
• SMILES: C1CC2=C(C1)N=C3C(=C2)C(=C(S3)C(=O)NC4=C(C=C(C=C4Br)F)F)N

DC_AC50 (CAT: I011671) is a chemical compound that acts as an inhibitor of copper-trafficking proteins Atox1 and CCS. It binds to purified Atox1 and CCS with dissociation constants (Kds) of 6.8 μM and 8.2 μM, respectively. In a FRET-based assay, at a concentration of 100 μM, it inhibits the interaction between Atox1 and domain 4 of the Cu+-ATPase ATP7B in the presence of zinc. In H1299 cells, at 10 μM concentration, DC_AC50 increases copper and reactive oxygen species (ROS) levels, reduces COX activity, and inhibits lipid biosynthesis. The compound shows concentration-dependent inhibition of proliferation in H1299, K562, MDA-MB-231, and 212LN cancer cells but has little effect on normal cells (PIG1, HDF, HaCaT, or MCF-10A). In an H1299 mouse xenograft model, DC_AC50 (100 mg/kg per day) reduces tumor growth.