For research use only. Not for therapeutic Use.
DFHBI-1T is a membrane-permeable RNA aptamers-activated fluorescence probe (ex/em=472 nm/507 nm). DFHBI-1T binds to RNA aptamers (Spinach, Spinach2, iSpinach, and Broccoli) and causes specific fluorescence and lower background fluorescence. DFHBI-1T is used to image RNA in live cells[1][2].
DFHBI-1T (20 μM; for 10 min) increases fluorescence in COS7 cells expressing (CGG)60-Spinach2 over DFHBI (20 μM)[1].
Broccoli-DFHBI-1T has ex/em=472 nm/507 nm and Spinach2-DFHBI-1T has ex/em=482 nm/505 nm[2].
| Catalog Number | I011255 |
| CAS Number | 1539318-36-9 |
| Synonyms | (5Z)-5-[(3,5-difluoro-4-hydroxyphenyl)methylidene]-2-methyl-3-(2,2,2-trifluoroethyl)imidazol-4-one |
| Molecular Formula | C13H9F5N2O2 |
| Purity | ≥95% |
| InChI | InChI=1S/C13H9F5N2O2/c1-6-19-10(12(22)20(6)5-13(16,17)18)4-7-2-8(14)11(21)9(15)3-7/h2-4,21H,5H2,1H3/b10-4- |
| InChIKey | AWYCLBWNRONMQC-WMZJFQQLSA-N |
| SMILES | CC1=NC(=CC2=CC(=C(C(=C2)F)O)F)C(=O)N1CC(F)(F)F |
| Reference | [1]. Wenjiao Song, et al. Plug-and-play fluorophores extend the spectral properties of Spinach. J Am Chem Soc. 2014 Jan 29;136(4):1198-201. [2]. Grigory S Filonov, et al. Broccoli: rapid selection of an RNA mimic of green fluorescent protein by fluorescence-based selection and directed evolution. J Am Chem Soc. 2014 Nov 19;136(46):16299-308. |
