Di-4-ANEPPS (Cat No.:R071994) is a membrane potential-sensitive fluorescent dye commonly used in electrophysiology and imaging studies. It intercalates into cellular membranes and exhibits fluorescence changes in response to alterations in membrane potential, making it a valuable tool for studying electrical activity in cells. Di-4-ANEPPS is particularly useful in monitoring action potentials and investigating cellular excitability in neurons, cardiomyocytes, and other excitable cells. Its high sensitivity and ability to provide real-time fluorescence data enable detailed analysis of membrane dynamics in live cell imaging and physiological research.