For research use only. Not for therapeutic Use.
DiBAC4(3) is a voltage-sensitive fluorescent dye (λex=490 nm, λem=505 nm).
The membrane hyperpolarization induced by 10 μM Evans blue (EB) in HEKBKα is clearly observed with DiBAC4(3), while the change in membrane potential (MP) by addition of 3 mM tetraethylammonium chloride (TEA) appears more slowly than that measured with microelectrode. The time to peak of hyperpolarization is 2.3±0.9 s (n=4) and 35.0±2.6 s (n=12, P<0.01) by the measurements with microelectrodes and DiBAC4(3), respectively[1].
| Catalog Number | R071998 |
| CAS Number | 70363-83-6 |
| Synonyms | 1,3-dibutyl-5-[(E)-3-(1,3-dibutyl-2,4,6-trioxo-1,3-diazinan-5-yl)prop-2-enylidene]-1,3-diazinane-2,4,6-trione |
| Molecular Formula | C27H40N4O6 |
| Purity | ≥95% |
| InChI | InChI=1S/C27H40N4O6/c1-5-9-16-28-22(32)20(23(33)29(26(28)36)17-10-6-2)14-13-15-21-24(34)30(18-11-7-3)27(37)31(25(21)35)19-12-8-4/h13-15,20H,5-12,16-19H2,1-4H3/b14-13+ |
| InChIKey | ATJCYSYHWGQAET-BUHFOSPRSA-N |
| SMILES | CCCCN1C(=O)C(C(=O)N(C1=O)CCCC)C=CC=C2C(=O)N(C(=O)N(C2=O)CCCC)CCCC |
| Reference | [1]. Yamada A, et al. Usefulness and limitation of DiBAC4(3), a voltage-sensitive fluorescent dye, for the measurement of membrane potentials regulated by recombinant large conductance Ca2+-activated K+ channels in HEK293 cells. Jpn J Pharmacol. 2001 Jul;86(3):342-50. |
