For research use only. Not for therapeutic Use.
DiFMUP is a fluorogenic substrate, and has been widely used for the continuous detection of phosphatase activities. DiFMUP is hydrolysis by a phosphatase results in the release of Xuorescent DIFMU, which can be easily followed in continuous mode by a Xuorescence reader[1][2].
DIFMUP (100 μM; 0-4 min) is hydrolyzed by protein tyrosine phosphatase 1B (PTP1B) in a time-dependent manner and the initial rate of the reaction velocity is increased with the enzyme concentration (30-600 ng/mL)[2].
| Catalog Number | R071577 |
| CAS Number | 214491-43-7 |
| Synonyms | (6,8-difluoro-4-methyl-2-oxochromen-7-yl) dihydrogen phosphate |
| Molecular Formula | C10H7F2O6P |
| Purity | ≥95% |
| InChI | InChI=1S/C10H7F2O6P/c1-4-2-7(13)17-9-5(4)3-6(11)10(8(9)12)18-19(14,15)16/h2-3H,1H3,(H2,14,15,16) |
| InChIKey | DZANYXOTJVLAEE-UHFFFAOYSA-N |
| SMILES | CC1=CC(=O)OC2=C(C(=C(C=C12)F)OP(=O)(O)O)F |
| Reference | [1]. Gee KR, et, al. Fluorogenic substrates based on fluorinated umbelliferones for continuous assays of phosphatases and beta-galactosidases. Anal Biochem. 1999 Aug 15;273(1):41-8. [2]. Welte S, et, al. 6,8-Difluoro-4-methylumbiliferyl phosphate: a fluorogenic substrate for protein tyrosine phosphatases. Anal Biochem. 2005 Mar 1;338(1):32-8. |
