DIGOXIGENIN NHS-ESTER

• CAT Number: M136951
• CAS Number: 129273-26-3
• Molecular Formula: C35H50N2O10
• Molecular Weight: 658.78
• Purity: ≥95%
• Storage: -20°C
• IUPAC Name: (2,5-dioxopyrrolidin-1-yl) 6-[[2-[[(3S,5R,8R,9S,10S,12R,13S,17R)-12,14-dihydroxy-10,13-dimethyl-17-(5-oxo-2H-furan-3-yl)-1,2,3,4,5,6,7,8,9,11,12,15,16,17-tetradecahydrocyclopenta[a]phenanthren-3-yl]oxy]acetyl]amino]hexanoate
• InChI: InChI=1S/C35H50N2O10/c1-33-13-11-23(45-20-28(39)36-15-5-3-4-6-31(42)47-37-29(40)9-10-30(37)41)17-22(33)7-8-25-26(33)18-27(38)34(2)24(12-14-35(25,34)44)21-16-32(43)46-19-21/h16,22-27,38,44H,3-15,17-20H2,1-2H3,(H,36,39)/t22-,23+,24-,25-,26+,27-,33+,34+,35?/m1/s1
• InChIKey: KHNDABJZSPPYLE-MZARVJLWSA-N
• SMILES: CC12CCC(CC1CCC3C2CC(C4(C3(CCC4C5=CC(=O)OC5)O)C)O)OCC(=O)NCCCCCC(=O)ON6C(=O)CCC6=O

DIGOXIGENIN NHS-ester（Cat No.:M136951） is a derivative of digoxigenin, a steroid found in the foxglove plant. It is used in molecular biology as a probe for nucleic acid detection and localization. The NHS-ester group reacts with primary amines on proteins, peptides, or nucleic acids, forming stable amide bonds. This conjugation allows for the specific labeling of target molecules, enabling their visualization or purification. Digoxigenin-labeled probes are commonly used in techniques such as in situ hybridization and Southern and Northern blotting. The high affinity of digoxigenin for anti-digoxigenin antibodies further enhances detection sensitivity.