For research use only. Not for therapeutic Use.
Dihydroethidium, also known as Hydroethidine and PD-MY 003, is a cell-permeable blue fluorescent dye. DHE-derived fluorescence at 570 nm is a convenient method for detection of intracellular and extracellular superoxide produced by phagocytic and vascular NADPH oxidase. Dihydroethidium is neuroprotective by reducing superoxide in mice after stroke.
| CAS Number | 104821-25-2 |
| Synonyms | Hydroethidine;PD-MY 003 |
| Molecular Formula | C21H21N3 |
| Purity | ≥95% |
| Storage | -20°C |
| InChI | InChI=1S/C21H21N3/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14/h3-13,21H,2,22-23H2,1H3 |
| InChIKey | XYJODUBPWNZLML-UHFFFAOYSA-N |
| SMILES | NC1=CC2=C(C3=C(C=C(N)C=C3)N(CC)C2C4=CC=CC=C4)C=C1 |
| Reference | </br>1:Measurement of Superoxide Production and NADPH Oxidase Activity by HPLC Analysis of Dihydroethidium Oxidation. Fernandes DC, Gonçalves RC, Laurindo FR.Methods Mol Biol. 2017;1527:233-249. doi: 10.1007/978-1-4939-6625-7_19. PMID: 28116721 </br>2:On the use of fluorescence lifetime imaging and dihydroethidium to detect superoxide in intact animals and ex vivo tissues: a reassessment. Michalski R, Michalowski B, Sikora A, Zielonka J, Kalyanaraman B.Free Radic Biol Med. 2014 Feb;67:278-84. doi: 10.1016/j.freeradbiomed.2013.10.816. Epub 2013 Nov 5. PMID: 24200598 Free PMC Article</br>3:Analysis of kinetics of dihydroethidium fluorescence with superoxide using xanthine oxidase and hypoxanthine assay. Chen J, Rogers SC, Kavdia M.Ann Biomed Eng. 2013 Feb;41(2):327-37. doi: 10.1007/s10439-012-0653-x. Epub 2012 Sep 11. PMID: 22965641 Free PMC Article</br>4:Assessment of superoxide production and NADPH oxidase activity by HPLC analysis of dihydroethidium oxidation products. Laurindo FR, Fernandes DC, Santos CX.Methods Enzymol. 2008;441:237-60. doi: 10.1016/S0076-6879(08)01213-5. Review. PMID: 18554538 </br>5:Analysis of dihydroethidium fluorescence for the detection of intracellular and extracellular superoxide produced by NADPH oxidase. Peshavariya HM, Dusting GJ, Selemidis S.Free Radic Res. 2007 Jun;41(6):699-712. PMID: 17516243 </br>6:Generation of superoxide anion by equine spermatozoa as detected by dihydroethidium. Burnaugh L, Sabeur K, Ball BA.Theriogenology. 2007 Feb;67(3):580-9. Epub 2006 Oct 12. PMID: 17045638 </br>7:Detection of intracellular superoxide formation in endothelial cells and intact tissues using dihydroethidium and an HPLC-based assay. Fink B, Laude K, McCann L, Doughan A, Harrison DG, Dikalov S.Am J Physiol Cell Physiol. 2004 Oct;287(4):C895-902. Epub 2004 Aug 11. PMID: 15306539 Free Article</br>8:Treatment with dihydroethidium reduces infarct size after transient focal cerebral ischemia in mice. Yu F, Sugawara T, Chan PH.Brain Res. 2003 Jul 18;978(1-2):223-7. PMID: 12834917 |
