For research use only. Not for therapeutic Use.
Dihydrofluorescein diacetate (Cat No.:I013220) is a commonly used fluorimetric probe for assessing oxidative stress levels. It is a cell-permeable compound that can be easily converted to the fluorescent dye, fluorescein, in the presence of reactive oxygen species (ROS). DHFDA is employed in both cell-free systems and cellular models to measure oxidative stress, as it provides a sensitive and quantitative method for evaluating the levels of ROS, which play a critical role in various physiological and pathological processes.
| Catalog Number | I013220 |
| CAS Number | 35340-49-9 |
| Molecular Formula | C₂₄H₁₈O₇ |
| Purity | ≥95% |
| Target | Dye Reagents |
| Solubility | DMSO |
| IUPAC Name | 2-(3,6-diacetyloxy-9H-xanthen-9-yl)benzoic acid |
| InChI | InChI=1S/C24H18O7/c1-13(25)29-15-7-9-19-21(11-15)31-22-12-16(30-14(2)26)8-10-20(22)23(19)17-5-3-4-6-18(17)24(27)28/h3-12,23H,1-2H3,(H,27,28) |
| InChIKey | YKSJJXGQHSESKB-UHFFFAOYSA-N |
| SMILES | CC(=O)OC1=CC2=C(C=C1)C(C3=C(O2)C=C(C=C3)OC(=O)C)C4=CC=CC=C4C(=O)O |
| Reference | [1]. Hempel SL, et al. Dihydrofluorescein diacetate is superior for detecting intracellular oxidants: comparison with 2//’,7//’-dichlorodihydrofluorescein diacetate, 5(and 6)-carboxy-2//’,7//’-dichlorodihydrofluorescein diacetate, and dihydrorhodamine 123. Free Radic Biol Med. 1999 Jul;27(1-2):146-59.<br>[2]. Diaz G, et al. Mitochondrial localization of reactive oxygen species by dihydrofluorescein probes. Histochem Cell Biol. 2003 Oct;120(4):319-25. <br>[3]. Aranda A, et al. Dichloro-dihydro-fluorescein diacetate (DCFH-DA) assay: a quantitative method for oxidative stress assessment of nanoparticle-treated cells. Toxicol In Vitro. 2013 Mar;27(2):954-63. |
