Disuccinimidyl sulfoxide

• CAT Number: R067696
• CAS Number: 1351828-03-9
• Molecular Formula: C14H16N2O9S
• Molecular Weight: 388.35
• Purity: ≥95%
• Synonyms: (2,5-dioxopyrrolidin-1-yl) 3-[3-(2,5-dioxopyrrolidin-1-yl)oxy-3-oxopropyl]sulfinylpropanoate
• InChI: InChI=1S/C14H16N2O9S/c17-9-1-2-10(18)15(9)24-13(21)5-7-26(23)8-6-14(22)25-16-11(19)3-4-12(16)20/h1-8H2
• InChIKey: XJSVVHDQSGMHAJ-UHFFFAOYSA-N
• SMILES: C1CC(=O)N(C1=O)OC(=O)CCS(=O)CCC(=O)ON2C(=O)CCC2=O

Disuccinimidyl sulfoxide (DSSO) (Cat No.: R067696) is a crosslinking reagent used in biochemical and molecular biology research to study protein-protein interactions, structure, and conformational changes. It features two succinimidyl ester groups, which react with amine groups on proteins, forming stable covalent bonds between them. DSSO is especially useful in applications like mass spectrometry-based proteomics, where it helps to capture transient protein interactions or complex formations. By crosslinking proteins, DSSO enables the identification of interaction sites and protein conformations in solution or cellular environments.

Reference

[1]. Kao A, et al., Development of a novel cross-linking strategy for fast and accurate identification of cross-linked peptides of protein complexes. Mol Cell Proteomics. 2011 Jan;10(1):M110.002212.
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