EACC

• CAT Number: I017944
• CAS Number: 864941-31-1
• Molecular Formula: C₁₃H₁₁N₃O₆S₂
• Molecular Weight: 369.37
• Purity: ≥95%
• Target: Autophagy
• IUPAC Name: ethyl N-[2-[(5-nitrothiophene-2-carbonyl)amino]thiophene-3-carbonyl]carbamate
• InChI: InChI=1S/C13H11N3O6S2/c1-2-22-13(19)15-10(17)7-5-6-23-12(7)14-11(18)8-3-4-9(24-8)16(20)21/h3-6H,2H2,1H3,(H,14,18)(H,15,17,19)
• InChIKey: ISLJZDYAPAUORR-UHFFFAOYSA-N
• SMILES: CCOC(=O)NC(=O)C1=C(SC=C1)NC(=O)C2=CC=C(S2)[N+](=O)[O-]

EACC(CAT: I017944) is a reversible autophagy inhibitor that blocks autophagic flux by selectively inhibiting the translocation of the autophagosome-specific SNARE protein, Stx17. This inhibition prevents the fusion of autophagosomes with lysosomes, effectively disrupting the final stage of autophagy. EACC uniquely inhibits Stx17 loading onto autophagosomes without affecting endo-lysosomal function, RABs, tethers, or lysosomal SNAREs, while also preventing their interaction with LC3 and Stx17. Widely used in cellular and molecular biology research, EACC is a valuable tool for studying autophagy mechanisms, intracellular trafficking, and the development of therapeutic strategies targeting autophagy in diseases such as cancer and neurodegeneration.