For research use only. Not for therapeutic Use.
Evans Blue (Direct Blue 53) is a potent inhibitor of L-glutamate uptake via the membrane bound excitatory amino acid transporter (EAAT). Evans Blue is a L-glutamate and kainate receptor-mediated currents inhibitor. Evans Blue has a strong affinity towards serum albumin, making it a high molecular weight protein tracer. Evans Blue is also used to study BBB (blood-brain barrier) permeability[1][2].
Evans blue, a bis-azo dye, is a non-permeating dye. In presence of plasma membrane damage, Evans blue enters in the cytoplasm and nucleus, thereby staining them blue. Evans blue is used for checking cell viability[1].
The amount of Evans Blue (2% solution; 4 mL/kg) stain accumulated in the brains of mice after ICH produced by collagenase injection was independent of the stain administration route[2].
| Catalog Number | A000007 |
| CAS Number | 314-13-6 |
| Synonyms | tetrasodium;4-amino-6-[[4-[4-[(8-amino-1-hydroxy-5,7-disulfonatonaphthalen-2-yl)diazenyl]-3-methylphenyl]-2-methylphenyl]diazenyl]-5-hydroxynaphthalene-1,3-disulfonate |
| Molecular Formula | C34H24N6Na4O14S4 |
| Purity | ≥95% |
| InChI | InChI=1S/C34H28N6O14S4.4Na/c1-15-11-17(3-7-21(15)37-39-23-9-5-19-25(55(43,44)45)13-27(57(49,50)51)31(35)29(19)33(23)41)18-4-8-22(16(2)12-18)38-40-24-10-6-20-26(56(46,47)48)14-28(58(52,53)54)32(36)30(20)34(24)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4 |
| InChIKey | ATNOAWAQFYGAOY-UHFFFAOYSA-J |
| SMILES | CC1=C(C=CC(=C1)C2=CC(=C(C=C2)N=NC3=C(C4=C(C=C3)C(=CC(=C4N)S(=O)(=O)[O-])S(=O)(=O)[O-])O)C)N=NC5=C(C6=C(C=C5)C(=CC(=C6N)S(=O)(=O)[O-])S(=O)(=O)[O-])O.[Na+].[Na+].[Na+].[Na+] |
| Reference | [1]. Linpeng Yao, et al. Evans Blue Dye: A Revisit of Its Applications in Biomedicine. Contrast Media Mol Imaging. 2018 Apr 22:2018:7628037. [2]. Anatol Manaenko, et al. Comparison Evans Blue injection routes: Intravenous versus intraperitoneal, for measurement of blood-brain barrier in a mice hemorrhage model. J Neurosci Methods. 2011 Feb 15;195(2):206-10. |
