Fibrinogen (Bovine)

• CAT Number: I043728
• CAS Number: 9001-32-5
• Purity: ≥95%

Fibrinogen (Bovine) is a selective proteolytic molecule that can be activated by thrombin to assemble fibrin clots. Fibrinogen can regulate the activation of NF-KB in endothelial cells and upregulate the expression of inflammatory chemokines MCP-1 and MCP-1. Fibrinogen plays a key role in blood clotting, thrombosis, atherosclerosis and the pathological development of venous grafts, and can be used in the study of blood clotting and vascular diseases[1][2][3].
Fibrinogen (0.3, 2, 4 mg/mL; 30 min) can induce a dose-dependent increase in the permeability of the endothelial cell layer to albumin, leading to the formation of gaps between endothelial cells and consequent albumin leakage[2].
Fibrinogen (2, 4 mg/mL; 1, 6, 12, 24 hours) increases leakage through the endothelial cell monolayer with time and dose[2].
Fibrinogen (4 mg/mL; 30 min) causes a dose-dependent increase in F-actin formation as well as gap formation in the endothelial cell monolayer[2].
Fibrinogen (2, 4 mg/mL; 30 min) can trigger a dose-dependent increase in ERK phosphorylation[2].
Fibrinogen (1000 µg/ml, 4 hours) can regulate the activation of NF-κB in endothelial cells (HUVEC) and specifically bind with the subunits p50, p65, c-Rel[3].
Fibrinogen (250 µg/mL, 4 hours) induces the activation of NF-κB, which is independent of Polybacterial B (HY-K1044) (5 µg/ml) and IL-1β (HY-P80503) (5 µg/ml)[3]
Fibrinogen (100, 250, 500, 1000 µg/mL; 4 hours) specifically and concentration-dependently upregulates the expression of MCP-1 and IL-8 mRNA in endothelial cells[3].

Reference

[1]. Brown JH, et al. The crystal structure of modified bovine fibrinogen. Proc Natl Acad Sci U S A. 2000;97(1):85-90.

[2]. Tyagi N, et al.Fibrinogen induces endothelial cell permeability. Mol Cell Biochem. 2008 Jan;307(1-2):13-22.
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[3]. Guo M, et al. Fibrinogen regulates the expression of inflammatory chemokines through NF-kappaB activation of endothelial cells. Thromb Haemost. 2004;92(4):858-866.
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