FlAsH-EDT2

• CAT Number: R052748
• CAS Number: 212118-77-9
• Molecular Formula: C24H18As2O5S4
• Molecular Weight: 664.486
• Purity: ≥95%
• Synonyms: 4’,5’-Bis(1,3,2-dithiarsolan-2-yl)-3’,6’-dihydroxyspiro[isobenzofuran-1(3H),9’-[9H]xanthen]-3-one; FlAsH; Fluorescein Arsenical Helix Binder; Lumio Green
• Storage: -20°C
• IUPAC Name: 4/',5/'-bis(1,3,2-dithiarsolan-2-yl)-3/',6/'-dihydroxyspiro[2-benzofuran-3,9/'-xanthene]-1-one
• InChI: InChI=1S/C24H18As2O5S4/c27-17-7-5-15-21(19(17)25-32-9-10-33-25)30-22-16(6-8-18(28)20(22)26-34-11-12-35-26)24(15)14-4-2-1-3-13(14)23(29)31-24/h1-8,27-28H,9-12H2
• InChIKey: AJNUQUGWNQHQDJ-UHFFFAOYSA-N
• SMILES: C1CS[As](S1)C2=C(C=CC3=C2OC4=C(C35C6=CC=CC=C6C(=O)O5)C=CC(=C4[As]7SCCS7)O)O

FlAsH-EDT2 (Fluorescein Arsenical Hairpin binder-2, or Fluorescein Arsenical Helix binder-2) is a biarsenical fluorescent dye used in molecular and cellular biology for the detection of specific protein motifs in living cells. It specifically binds to tetracysteine (Cys-Cys-Xaa-Xaa-Cys-Cys) sequences engineered into proteins of interest. Upon binding to these motifs, FlAsH-EDT2 emits a green fluorescent signal, allowing real-time visualization of protein localization, trafficking, and interactions in live cells. The use of FlAsH-EDT2 is particularly valuable in studying dynamic cellular processes because it provides a non-invasive method for labeling and tracking proteins without disrupting their function. However, due to its arsenic content, careful handling and experimental controls are required.