Florfenicol

• CAT Number: A000832
• CAS Number: 73231-34-2
• Molecular Formula: C12H14Cl2FNO4S
• Molecular Weight: 358.20
• Purity: ≥95%
• Synonyms: 73231-34-2; Nuflor; (-)-Florfenicol; Sch-25298; Nuflor gold
• Target: Bacterial
• Storage: -20°C
• IUPAC Name: 2,2-dichloro-N-[(1R,2S)-3-fluoro-1-hydroxy-1-(4-methylsulfonylphenyl)propan-2-yl]acetamide
• InChI: InChI=1S/C12H14Cl2FNO4S/c1-21(19,20)8-4-2-7(3-5-8)10(17)9(6-15)16-12(18)11(13)14/h2-5,9-11,17H,6H2,1H3,(H,16,18)/t9-,10-/m1/s1
• InChIKey: AYIRNRDRBQJXIF-NXEZZACHSA-N
• SMILES: CS(=O)(=O)C1=CC=C(C=C1)[C@H]([C@@H](CF)NC(=O)C(Cl)Cl)O

Florfenicol（Cat No.:A000832）is a broad-spectrum antibiotic primarily used in veterinary medicine to treat respiratory, enteric, and systemic infections in livestock and aquaculture. As a synthetic derivative of thiamphenicol, it belongs to the amphenicol class and works by inhibiting bacterial protein synthesis, targeting the 50S ribosomal subunit. Florfenicol is effective against a range of gram-positive and gram-negative bacteria, including strains resistant to other antibiotics. Due to its favorable pharmacokinetics and reduced risk of inducing resistance, it is valuable in agricultural settings and is studied for antimicrobial efficacy and resistance mechanisms.

Reference

1: Ling Z, Yonghong L, Junfeng L, Li Z, Xianqiang L. Tilmicosin- and
florfenicol-loaded hydrogenated castor oil-solid lipid nanoparticles to pigs:
Combined antibacterial activities and pharmacokinetics. J Vet Pharmacol Ther.
2017 Nov 15. doi: 10.1111/jvp.12465. [Epub ahead of print] PubMed PMID: 29139136.

2: Fan R, Li D, Feßler AT, Wu C, Schwarz S, Wang Y. Distribution of optrA and cfr
in florfenicol-resistant Staphylococcus sciuri of pig origin. Vet Microbiol. 2017
Oct;210:43-48. doi: 10.1016/j.vetmic.2017.07.030. Epub 2017 Sep 1. PubMed PMID:
29103695.

3: Hu D, Cao S, Zhang G, Xiao Y, Liu S, Shang Y. Florfenicol-induced
Mitochondrial Dysfunction Suppresses Cell Proliferation and Autophagy in
Fibroblasts. Sci Rep. 2017 Oct 19;7(1):13554. doi: 10.1038/s41598-017-13860-9.
PubMed PMID: 29051574; PubMed Central PMCID: PMC5648778.

4: Li Y, Li Y, Fernandez Crespo R, Leanse LG, Langford PR, Bossé JT.
Characterization of the Actinobacillus pleuropneumoniae SXT-related integrative
and conjugative element ICEApl2 and analysis of the encoded FloR protein:
hydrophobic residues in transmembrane domains contribute dynamically to
florfenicol and chloramphenicol efflux. J Antimicrob Chemother. 2017 Oct 6. doi:
10.1093/jac/dkx342. [Epub ahead of print] PubMed PMID: 29029160.

5: Yang J, Sun G, Qian M, Huang L, Ke X, Yang B. Development of a
high-performance liquid chromatography method for the determination of
florfenicol in animal feedstuffs. J Chromatogr B Analyt Technol Biomed Life Sci.
2017 Nov 15;1068-1069:9-14. doi: 10.1016/j.jchromb.2017.09.045. Epub 2017 Sep 29.
PubMed PMID: 29028619.

6: Cao Z, Liu X, Xu J, Zhang J, Yang Y, Zhou J, Xu X, Lowry GV. Removal of
Antibiotic Florfenicol by Sulfide-Modified Nanoscale Zero-Valent Iron. Environ
Sci Technol. 2017 Oct 3;51(19):11269-11277. doi: 10.1021/acs.est.7b02480. Epub
2017 Sep 25. PubMed PMID: 28902992.

7: Imran M, Fazal-E-Habib, Tawab A, Rauf W, Rahman M, Khan QM, Asi MR, Iqbal M.
LC-MS/MS based method development for the analysis of florfenicol and its
application to estimate relative distribution in various tissues of broiler
chicken. J Chromatogr B Analyt Technol Biomed Life Sci. 2017 Sep 15;1063:163-173.
doi: 10.1016/j.jchromb.2017.08.029. Epub 2017 Aug 24. PubMed PMID: 28866358.

8: Budd C, Flanagan C, Gillett A, Hanger J, Loader JJ, Govendir M. Assessment of
florfenicol as a possible treatment for chlamydiosis in koalas (Phascolarctos
cinereus). Aust Vet J. 2017 Sep;95(9):343-349. doi: 10.1111/avj.12617. PubMed
PMID: 28845567.

9: Jackson AE. In this issue – September 2017: Identifying bloodlines of animals
with mutations · Sexed semen in dairy cows · Conventional vs automatic milking
systems · Overground endoscopy in Thoroughbred horses · Honeys for wound
healing · Cabergoline in anoestrous bitches · Florfenicol not recommended for
chlamydiosis in koalas. Aust Vet J. 2017 Sep;95(9):305-306. doi:
10.1111/avj.12632. PubMed PMID: 28845563.

10: Batrawi N, Naseef H, Al-Rimawi F. Development and Validation of a
Stability-Indicating HPLC Method for the Simultaneous Determination of
Florfenicol and Flunixin Meglumine Combination in an Injectable Solution. J Anal
Methods Chem. 2017;2017:1529280. doi: 10.1155/2017/1529280. Epub 2017 Jul 11.
PubMed PMID: 28781907; PubMed Central PMCID: PMC5525076.