Fluo-4 AM

• CAT Number: I013074
• CAS Number: 273221-67-3
• Molecular Formula: C₅₁H₅₀F₂N₂O₂₃
• Molecular Weight: 1096.94
• Purity: ≥95%
• Target: Dye Reagents
• Solubility: DMSO
• IUPAC Name: acetyloxymethyl 2-[2-[2-[5-[3-(acetyloxymethoxy)-2,7-difluoro-6-oxoxanthen-9-yl]-2-[bis[2-(acetyloxymethoxy)-2-oxoethyl]amino]phenoxy]ethoxy]-N-[2-(acetyloxymethoxy)-2-oxoethyl]-4-methylanilino]acetate
• InChI: InChI=1S/C51H50F2N2O23/c1-28-7-9-39(54(19-47(62)74-24-69-30(3)57)20-48(63)75-25-70-31(4)58)45(13-28)66-11-12-67-46-14-34(8-10-40(46)55(21-49(64)76-26-71-32(5)59)22-50(65)77-27-72-33(6)60)51-35-15-37(52)41(61)17-42(35)78-43-18-44(38(53)16-36(43)51)73-23-68-29(2)56/h7-10,13-18H,11-12,19-27H2,1-6H3
• InChIKey: QOMNQGZXFYNBNG-UHFFFAOYSA-N
• SMILES: CC1=CC(=C(C=C1)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)OCCOC2=C(C=CC(=C2)C3=C4C=C(C(=O)C=C4OC5=CC(=C(C=C53)F)OCOC(=O)C)F)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C

Fluo-4 AM (Cat.No:I013074) is a fluorescent calcium indicator commonly used in cell imaging and calcium ion measurement studies. It is a cell-permeable dye that can be easily loaded into cells, where it binds to intracellular calcium ions. Fluo-4 AM enables the visualization and quantification of intracellular calcium dynamics, providing valuable insights into cellular processes and signaling pathways.

Reference

[1]. Gee KR, et al. Chemical and physiological characterization of fluo-4 Ca(2+)-indicator dyes. Cell Calcium. 2000 Feb;27(2):97-106.