Fluorescein-5-thiosemicarbazide

• CAT Number: I043289
• CAS Number: 76863-28-0
• Molecular Formula: C21H15N3O5S
• Molecular Weight: 421.43
• Purity: ≥95%
• Synonyms: 1-amino-3-(3′,6′-dihydroxy-3-oxospiro[2-benzofuran-1,9′-xanthene]-5-yl)thiourea
• IUPAC Name: 5-(aminocarbamothioylamino)-2-(3-hydroxy-6-oxoxanthen-9-yl)benzoic acid
• InChI: InChI=1S/C21H15N3O5S/c22-24-21(30)23-10-1-4-13(16(7-10)20(27)28)19-14-5-2-11(25)8-17(14)29-18-9-12(26)3-6-15(18)19/h1-9,25H,22H2,(H,27,28)(H2,23,24,30)
• InChIKey: MEUCHQDLZYLNQY-UHFFFAOYSA-N
• SMILES: C1=CC(=C(C=C1NC(=S)NN)C(=O)O)C2=C3C=CC(=O)C=C3OC4=C2C=CC(=C4)O

Fluorescein-5-thiosemicarbazide（Cat No.:I043289）is a derivative of fluorescein, a widely used fluorescent dye, with a thiosemicarbazide group attached at the 5-position. The thiosemicarbazide group enhances the molecule’s reactivity, enabling it to participate in chemical reactions such as covalent binding with biomolecules or detecting specific chemical species. Fluorescein-5-thiosemicarbazide is commonly used in biological research for labeling and imaging purposes, particularly in fluorescence microscopy and flow cytometry. Its bright fluorescence allows for sensitive detection of biomolecular interactions, making it useful in studies of cellular processes, enzyme activity, and the localization of specific proteins or nucleic acids.