Fluorescein isothiocyanate

• CAT Number: I027601
• CAS Number: 25168-13-2
• Molecular Formula: C21H12NO5S-
• Molecular Weight: 390.39
• Purity: 98%
• Synonyms: Fluorescein isothiocyanate
• Solubility: Soluble in DMSO
• Appearance: Solid powder
• Storage: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
• IUPAC Name: Benzoic acid, 2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)-, monoisothiocyanato deriv.
• InChI: InChI=1S/C20H12O5.CNS/c21-11-5-7-15-17(9-11)24-18-10-12(22)6-8-16(18)20(15)14-4-2-1-3-13(14)19(23)25-20;2-1-3/h1-10,21-22H;/q;-1
• InChIKey: UAVRVUMOUGGZES-UHFFFAOYSA-N
• SMILES: Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5ccccc45)c1.[N-]=C=S

Fluorescein isothiocyanate is a colorimetric probe formed by the reaction of adipic acid dihydrazide and fluorescein isothiocyanate.

Reference

1. Zhang L, Zhang X. A selectively fluorescein-based colorimetric probe for detecting copper(II) ion. Spectrochim Acta A Mol Biomol Spectrosc. 2014 Dec 10;133:54-9. doi: 10.1016/j.saa.2014.04.130. Epub 2014 Apr 30. PMID: 24929315.
2. Ahn B, Rhee SG, Stadtman ER. Use of fluorescein hydrazide and fluorescein thiosemicarbazide reagents for the fluorometric determination of protein carbonyl groups and for the detection of oxidized protein on polyacrylamide gels. Anal Biochem. 1987 Mar;161(2):245-57. doi: 10.1016/0003-2697(87)90448-9. PMID: 2883911.
3. Zhang J, Zhang L, Wei Y, Ma J, Shuang S, Cai Z, Dong C. A selectively fluorescein-based colorimetric probe for detecting copper(II) ion. Spectrochim Acta A Mol Biomol Spectrosc. 2014 Mar 25;122:731-6. doi: 10.1016/j.saa.2013.11.096. Epub 2013 Nov 23. PMID: 24370938.