Fluorescein isothiocyanate

• CAT Number: I027601
• CAS Number: 25168-13-2
• Molecular Formula: C21H12NO5S-
• Molecular Weight: 390.39
• Purity: 98%
• Synonyms: Fluorescein isothiocyanate
• Solubility: Soluble in DMSO
• Appearance: Solid powder
• Storage: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
• IUPAC Name: Benzoic acid, 2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)-, monoisothiocyanato deriv.
• InChI: InChI=1S/C20H12O5.CNS/c21-11-5-7-15-17(9-11)24-18-10-12(22)6-8-16(18)20(15)14-4-2-1-3-13(14)19(23)25-20;2-1-3/h1-10,21-22H;/q;-1
• InChIKey: UAVRVUMOUGGZES-UHFFFAOYSA-N
• SMILES: Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5ccccc45)c1.[N-]=C=S

Fluorescein Isothiocyanate (CAT: I027601) is a widely used fluorescent labeling reagent that covalently binds to primary amines in proteins, peptides, and other biomolecules through its isothiocyanate functional group. FITC emits strong green fluorescence (maximum emission ~520 nm) upon excitation, making it ideal for applications in immunofluorescence, flow cytometry, and fluorescence microscopy. Its high photostability and compatibility with various biological assays enable the visualization and quantification of biomolecular interactions and cellular processes. FITC remains a cornerstone in molecular biology and biomedical research, facilitating advanced studies in diagnostics, cell signaling, and bioimaging.