For research use only. Not for therapeutic Use.
Fluorescein tyramide is a green fluorescent reagent (λabs: 494 nm; λem: 517 nm). Fluorescein tyramide is widely used for tyramide signal amplification (TSA) with a low-abundance in IHC, ICC, in situ hybridization (FISH) and flow cytometry (FCM) applications[1][2].
HRP catalyzes localized deposition of multiple tyramide molecules (catalyzed reporter deposition, CARD), binding the fluorescein tyramide to adjacent tyrosines to enhance fluorescent signal.
| Catalog Number | I045475 |
| CAS Number | 210236-90-1 |
| Synonyms | 3′,6′-dihydroxyspiro[2-benzofuran-3,9′-xanthene]-1-one;N-[2-(4-hydroxyphenyl)ethyl]acetamide |
| Molecular Formula | C29H21NO7 |
| Purity | ≥95% |
| InChI | InChI=1S/C20H12O5.C10H13NO2/c21-11-5-7-15-17(9-11)24-18-10-12(22)6-8-16(18)20(15)14-4-2-1-3-13(14)19(23)25-20;1-8(12)11-7-6-9-2-4-10(13)5-3-9/h1-10,21-22H;2-5,13H,6-7H2,1H3,(H,11,12) |
| InChIKey | RIHCPYWHAYBEHO-UHFFFAOYSA-N |
| SMILES | CC(=O)NCCC1=CC=C(C=C1)O.C1=CC=C2C(=C1)C(=O)OC23C4=C(C=C(C=C4)O)OC5=C3C=CC(=C5)O |
| Reference | [1]. A H Hopman, et al. Rapid synthesis of biotin-, digoxigenin-, trinitrophenyl-, and fluorochrome-labeled tyramides and their application for In situ hybridization using CARD amplification. J Histochem Cytochem. 1998 Jun;46(6):771-7. [2]. R P van Gijlswijk, et al. Fluorochrome-labeled tyramides: use in immunocytochemistry and fluorescence in situ hybridization. J Histochem Cytochem. 1997 Mar;45(3):375-82. |
