Fluorescent Substrate for Glu-Specific Proteases

• CAT Number: M096607
• CAS Number: 143147-75-5
• Molecular Formula: C63H73N11O18
• Molecular Weight: 1272.32
• Purity: ≥95%
• Synonyms: (2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[(2-aminobenzoyl)amino]propanoyl]amino]-3-phenylpropanoyl]amino]propanoyl]amino]-3-phenylpropanoyl]amino]-4-carboxybutanoyl]amino]-3-methylbutanoyl]amino]-3-phenylpropanoyl]amino]-3-(4-hydroxy-3-nitrophenyl)propanoyl]amino]butanedioic acid
• InChI: InChI=1S/C63H73N11O18/c1-34(2)53(62(88)71-46(30-39-20-12-7-13-21-39)60(86)70-47(61(87)72-48(63(89)90)33-52(78)79)31-40-24-26-50(75)49(32-40)74(91)92)73-57(83)43(25-27-51(76)77)67-59(85)45(29-38-18-10-6-11-19-38)69-55(81)36(4)66-58(84)44(28-37-16-8-5-9-17-37)68-54(80)35(3)65-56(82)41-22-14-15-23-42(41)64/h5-24,26,32,34-36,43-48,53,75H,25,27-31,33,64H2,1-4H3,(H,65,82)(H,66,84)(H,67,85)(H,68,80)(H,69,81)(H,70,86)(H,71,88)(H,72,87)(H,73,83)(H,76,77)(H,78,79)(H,89,90)/t35-,36-,43-,44-,45-,46-,47-,48-,53-/m0/s1
• InChIKey: GGFWDXWGSVJEBX-XMJIKGBQSA-N
• SMILES: CC(C)C(C(=O)NC(CC1=CC=CC=C1)C(=O)NC(CC2=CC(=C(C=C2)O)[N+](=O)[O-])C(=O)NC(CC(=O)O)C(=O)O)NC(=O)C(CCC(=O)O)NC(=O)C(CC3=CC=CC=C3)NC(=O)C(C)NC(=O)C(CC4=CC=CC=C4)NC(=O)C(C)NC(=O)C5=CC=CC=C5N

Fluorescent Substrate for Glu-Specific Proteases is a V8 protease-Specific chromogenic substrate[1].
V8 protease (0, 0.1, 0.5, 1.0, 5.0, 10, 20 µg/ml) is assayed with a solution of 200 µM of the synthetic substrate (Fluorescent Substrate for Glu-Specific Proteases; ABz-AFAFEVFY(NO2)D-OH) in HEPES buffer pH 7.4 and at room temperature conditions. The fluorescence was read each 1 min for 60 min by fluorescence microplate reader[1].

Reference

[1]. Qudsi, et al. Development of a novel approach to influence the secretion of pathogenicity factors of Staphylococcus aureus by synthetic peptides. Fr., 04. Mai. 2012.