For research use only. Not for therapeutic Use.
Flutax-2 (5/6-mixture) is an active fluorescent derivative of paclitaxel. Flutax-2 (5/6-mixture) binds to a polymerized α,β tubulin dimer. Excitation/emission wavelength: 496/524 nm. Paclitaxel, a diterpenoid secondary metabolite produced by Taxus species, can be used for the research of a variety of cancers[1].
Preparation of Flutax-2 working solution
1.1 Preparation of the stock solution
Dissolve 10 mg of Flutax-2 in 1 mL of DMSO.
Note: It is recommended to store the stock solution at -20℃or -80℃away from light and avoid repetitive freeze-thaw cycles.
1.2 Preparation of Flutax-2 working solution
Dilute the stock solution in sacetate buffer to obtain 100 nM-1 μM of Flutax-2 working solution.
Note: Please adjust the concentration of Flutax-2 working solution according to the actual situation.
Cell staining
2.1 For suspension cells: Centrifuge at 1000 g at 4℃for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4℃for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
2.2 Add 1 mL of Flutax-2 working solution, and then incubate at room temperature for 10-30 minutes.
2.3 Centrifuge at 400 g at 4℃for 3-4 minutes and then discard the supernatant.
2.4 Wash twice with PBS, 5 minutes each time.
2.5 Resuspend cells with serum-free cell culture medium or PBS, and then detect by fluorescence microscope or flow cytometer.
Storage
-20℃, 1 year
Protect from light
| Catalog Number | I045860 |
| Synonyms | [(1S,2S,3R,4S,7R,9S,10S,12R,15S)-4,12-diacetyloxy-15-[(2R,3S)-3-benzamido-2-hydroxy-3-phenylpropanoyl]oxy-9-[(2S)-2-[(2′,7′-difluoro-3′,6′-dihydroxy-3-oxospiro[2-benzofuran-1,9′-xanthene]-5-carbonyl)amino]propanoyl]oxy-1-hydroxy-10,14,17,17-tetramethyl-11-oxo-6-oxatetracyclo[11.3.1.03,10.04,7]heptadec-13-en-2-yl] benzoate |
| Molecular Formula | C142H128F4N4O42 |
| Purity | ≥95% |
| InChI | InChI=1S/C71H64F2N2O21/c1-33-51(92-66(87)56(80)55(37-17-11-8-12-18-37)75-61(82)38-19-13-9-14-20-38)31-70(88)60(94-64(85)39-21-15-10-16-22-39)58-68(7,59(81)57(90-35(3)76)54(33)67(70,5)6)52(30-53-69(58,32-89-53)95-36(4)77)93-63(84)34(2)74-62(83)40-23-24-42-41(25-40)65(86)96-71(42)43-26-45(72)47(78)28-49(43)91-50-29-48(79)46(73)27-44(50)71/h8-29,34,51-53,55-58,60,78-80,88H,30-32H2,1-7H3,(H,74,83)(H,75,82)/t34-,51-,52-,53+,55-,56+,57+,58-,60-,68+,69-,70+/m0/s1 |
| InChIKey | PGOSVWLSFOGNCE-LIESBUPCSA-N |
| SMILES | CC1=C2C(C(=O)C3(C(CC4C(C3C(C(C2(C)C)(CC1OC(=O)C(C(C5=CC=CC=C5)NC(=O)C6=CC=CC=C6)O)O)OC(=O)C7=CC=CC=C7)(CO4)OC(=O)C)OC(=O)C(C)NC(=O)C8=CC9=C(C=C8)C1(C2=CC(=C(C=C2OC2=CC(=C(C=C21)F)O)O)F)OC9=O)C)OC(=O)C |
| Reference | [1]. P B Vieira, et al. Analysis of microtubule cytoskeleton distribution using a fluorescent taxoid in two trichomonadid protozoa: Trichomonas gallinae and Tritrichomonas foetus. Exp Parasitol. 2008 May;119(1):186-91. [2]. P B Vieira, et al. Analysis of microtubule cytoskeleton distribution using a fluorescent taxoid in two trichomonadid protozoa: Trichomonas gallinae and Tritrichomonas foetus. Exp Parasitol. 2008 May;119(1):186-91. |
