G418 Sulfate

• CAT Number: I000364
• CAS Number: 108321-42-2
• Molecular Formula: C₂₀H₄₀N₄O₁₀. 2 H₂SO₄
• Molecular Weight: 692.71
• Purity: ≥95%
• Synonyms: 2-[4,6-diamino-3-[3-amino-4,5-dihydroxy-6-(1-hydroxyethyl)oxan-2-yl]oxy-2-hydroxycyclohexyl]oxy-5-methyl-4-(methylamino)oxane-3,5-diol;sulfuric acid
• Target: Bacterial
• Solubility: H2O: ＞ 30 mg/mL
• IUPAC Name: (2S,3S,4S,5S)-2-[(1S,2S,3R,4S,6R)-4,6-diamino-3-[(2S,3R,4R,5S,6R)-3-amino-4,5-dihydroxy-6-(1-hydroxyethyl)oxan-2-yl]oxy-2-hydroxycyclohexyl]oxy-5-methyl-4-(methylamino)oxane-3,5-diol;sulfuric acid
• InChI: InChI=1S/C20H40N4O10.2H2O4S/c1-6(25)14-11(27)10(26)9(23)18(32-14)33-15-7(21)4-8(22)16(12(15)28)34-19-13(29)17(24-3)20(2,30)5-31-19;2*1-5(2,3)4/h6-19,24-30H,4-5,21-23H2,1-3H3;2*(H2,1,2,3,4)/t6?,7-,8+,9+,10+,11-,12-,13-,14+,15+,16-,17-,18+,19-,20+;;/m0../s1
• InChIKey: UHEPSJJJMTWUCP-NKCAIAFTSA-N
• SMILES: CC([C@@H]1[C@H]([C@@H]([C@H]([C@H](O1)O[C@@H]2[C@H](C[C@H]([C@@H]([C@H]2O)O[C@H]3[C@H]([C@@H]([C@](CO3)(C)O)NC)O)N)N)N)O)O)O.OS(=O)(=O)O.OS(=O)(=O)O

G418 Sulfate（Cat No.:I000364）is an aminoglycoside antibiotic commonly used as a selection agent in molecular biology, particularly for eukaryotic cells. It functions by inhibiting protein synthesis, leading to cell death in cells that lack resistance. G418 is effective against both Gram-positive and Gram-negative bacteria, as well as various eukaryotic cells, making it ideal for selecting genetically modified cells with neomycin-resistance genes (such as neo or kan genes). Widely applied in gene-editing experiments and cell line development, G418 Sulfate is essential for creating stable cell lines in biomedical research.

Reference

[1]. G-418, From Wikipedia