For research use only. Not for therapeutic Use.
CleanCap Reagent GG (3ʹOMe) is designed for the cotranscriptional capping of mRNA to produce an mRNA with naturally occurring Cap 1. Cap 1 mRNAs have superior in vivo activity compared to Cap 0 mRNA produced by legacy capping methods such as mCap or anti-reverse cap analog (ARCA).<br />
CleanCap GG (3' OMe) includes the 5' N7-Methyl-3'-O-Methylguanosine commonly found in mRNA capped using ARCA, whereas CleanCap GG (N-7133) has a naturally occurring 5' N7-Methylguanosine structure.<br />
CleanCap can be used in conjunction with TriLink's catalog of modified NTPs.<br />
Using the recommended conditions, transcription with CleanCap GG (3’OMe) results in 70-90% capped material, generating a Cap 1 structure and gives crude yields of ~1.5 mg per mL of transcription. Final capping is dependent upon the DNA template and final mRNA sequence. Secondary structure due to RNA length and base composition can affect final capping efficiency.
| Catalog Number | R074748 |
| Molecular Formula | C33H45N15O25P4 (free acid) |
| Purity | 95% |
| Analysis method | AX-HPLC |
