For research use only. Not for therapeutic Use.
G13KS is a deubiquitinase UCHL1 ligand and inhibitor. G13KS inhibits recombinant and cellular UCHL1. G13KS reduces levels of monoubiquitin in human glioblastoma cells[1]. GK13S is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.
GK13S (0-1 μM, 1 h) inhibits recombinant UCHL1 with an IC50 of 50 nM[1].
GK13S (1-10 μM, 24 h) inhibits cellular UCHL1 in HEK293 cells[1].
GK13S (5 μM, 72 h) does not impair HEK293 cell growth[1].
GK13S (5 μM, 48 h) reduces ubiquitin levels in U-87 MG cells[1].
| Catalog Number | I041218 |
| Synonyms | (3S)-1-cyano-N-[1-[4-(pent-4-ynylcarbamoyl)phenyl]imidazol-4-yl]pyrrolidine-3-carboxamide |
| Molecular Formula | C21H22N6O2 |
| Purity | ≥95% |
| InChI | InChI=1S/C21H22N6O2/c1-2-3-4-10-23-20(28)16-5-7-18(8-6-16)27-13-19(24-15-27)25-21(29)17-9-11-26(12-17)14-22/h1,5-8,13,15,17H,3-4,9-12H2,(H,23,28)(H,25,29)/t17-/m0/s1 |
| InChIKey | LSHAYFDZIOIKII-KRWDZBQOSA-N |
| SMILES | C#CCCCNC(=O)C1=CC=C(C=C1)N2C=C(N=C2)NC(=O)C3CCN(C3)C#N |
| Reference | [1]. Grethe C, et al. Structural basis for specific inhibition of the deubiquitinase UCHL1. Nat Commun. 2022 Oct 10;13(1):5950. |
