GKI-1

• CAT Number: I019648
• CAS Number: 2444764-03-6
• Molecular Formula: C₁₅H₁₂ClN₃
• Molecular Weight: 269.73
• Purity: ≥95%
• IUPAC Name: N-(4-chlorophenyl)-3-(1H-pyrazol-4-yl)aniline
• InChI: InChI=1S/C15H12ClN3/c16-13-4-6-14(7-5-13)19-15-3-1-2-11(8-15)12-9-17-18-10-12/h1-10,19H,(H,17,18)
• InChIKey: GPXJDHFWTOJHQQ-UHFFFAOYSA-N
• SMILES: C1=CC(=CC(=C1)NC2=CC=C(C=C2)Cl)C3=CNN=C3

GKI-1(CAT: I019648) is a selective inhibitor of Greatwall kinase (GWL), with IC₅₀ values of 4.9 µM and 2.5 µM against full-length human GWL (hGWLFL) and the kinase domain (hGWL-KinDom), respectively. This compound demonstrates robust inhibitory activity against ROCK1 (IC₅₀: 11 µM) but exhibits minimal effects on PKA, highlighting its specificity. GKI-1 effectively inhibits GWL activity in cellular systems, making it a valuable tool for studying GWL-mediated regulatory pathways, particularly in cell cycle progression and mitotic regulation. Its efficacy provides insights into kinase-targeted therapeutic strategies, particularly in cancer and proliferative disorders.