Gly-AMC HCl

• CAT Number: I028124
• CAS Number: 208459-17-0
• Molecular Formula: C12H13ClN2O3
• Molecular Weight: 268.69
• Purity: 98%
• Synonyms: Gly-AMC HCl; H-Gly-AMC HCl; L-Gly-7-Amino-4-Methylcoumarin; H-Gly-AMC; ; ZINC57339; AK144290; DS-7861; DA-02879; FT-0698956
• Solubility: Soluble in DMSO
• Appearance: Solid powder
• Storage: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
• IUPAC Name: Acetamide, 2-amino-N-(4-methyl-2-oxo-2H-1-benzopyran-7-yl)-, monohydrochloride
• InChI: InChI=1S/C12H12N2O3.ClH/c1-7-4-12(16)17-10-5-8(2-3-9(7)10)14-11(15)6-13;/h2-5H,6,13H2,1H3,(H,14,15);1H
• InChIKey: DGTMMNCLSKYGIF-UHFFFAOYSA-N
• SMILES: O=C(NC1=CC=C(C(O2)=C1)C(C)=CC2=O)CN.[H]Cl

Gly-AMC HCl (Glycyl-7-amido-4-methylcoumarin hydrochloride) (Cat No.: I028124) is a fluorogenic substrate commonly used in enzymatic assays to measure protease activity, particularly aminopeptidases. Upon enzymatic cleavage, it releases 7-amino-4-methylcoumarin (AMC), a fluorescent molecule detectable by fluorescence spectroscopy. This property makes it ideal for high-throughput screening and kinetic studies in biochemical and pharmaceutical research. Gly-AMC HCl is widely used to study enzyme specificity, inhibitor screening, and metabolic processes involving peptide cleavage, contributing valuable insights into enzyme function and regulation.