Green DND-26

• CAT Number: I043814
• CAS Number: 220524-71-0
• Molecular Formula: C18H25BF2N4O
• Molecular Weight: 362.23
• Purity: ≥95%

Green DND-26 is a green fluorescently labeled lysosomal probe with a maximum excitation/emission wavelength of 504/511 nm. The structure is composed of a fluorescein group and linked weak bases, which can freely cross the cell membrane and generally gather on spherical organelles. Green DND-26 is suitable for observing the internal biosynthesis and related pathogenesis of lysosomes[1].
Preparation of Green DND-26 working solution
1. Preparation of the stock solution
1.1 Restore the solution to the room temperature and concentrate it to the bottom of the tube by instantaneous centrifugation.
1.2 Dilute 1 mM storage solution to the working solution using medium or appropriate buffer (such as PBS) . The recommended working solution is 50-100 nM.
Note: Please adjust the concentration of Green DND-26 working solution according to the actual situation, and use it now.
Cell staining
2.1 For suspension cells: Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
2.2 Add 1 mL of Green DND-26 working solution, and then incubate at room temperature for 30 minutes.
2.3 Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
2.4 Wash twice with PBS, 5 minutes each time.
2.5 Resuspend cells with serum-free cell culture medium or PBS, and then detect by fluorescence microscope or flow cytometer.
Storage
-20°C save, Protect from light

Reference

[1]. Sun B, et al. Acid-Activatable Transmorphic Peptide-Based Nanomaterials for Photodynamic Therapy. Angew Chem Int Ed Engl. 2020;59(46):20582-20588.
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[2]. Van der Velden JL, et al. LysoTracker is a marker of differentiated alveolar type II cells. Respir Res. 2013;14(1):123. Published 2013 Nov 11.
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