For research use only. Not for therapeutic Use.
Guanosine 5′-triphosphate-5′-adenosine (GpppA) triammonium, a 5′ cap analog, can be used for RNA synthesis in vitro. Guanosine 5′-triphosphate-5′-adenosine triammonium is a fluorescent substrate analog[1][2].
Guanosine 5′-triphosphate-5′-adenosine (GpppA) triammonium labeled with pyrene at the 3′-O position of adenosine acts as an artificial substrate. Fluorescently labeled GpppA triammonium and GpppG analogs as potential substrates that represent a reasonable compromise between the structural complexity and requirements of the enzyme[1].
| Catalog Number | I040114 |
| Synonyms | [[(2R,4S,5R)-5-(2-amino-6-oxo-1H-purin-9-yl)-3,4-dihydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl] [[(2R,4S,5R)-5-(6-aminopurin-9-yl)-3,4-dihydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl] hydrogen phosphate;azane |
| Molecular Formula | C20H36N13O17P3 |
| Purity | ≥95% |
| InChI | InChI=1S/C20H27N10O17P3.3H3N/c21-14-8-15(24-3-23-14)29(4-25-8)18-12(33)10(31)6(44-18)1-42-48(36,37)46-50(40,41)47-49(38,39)43-2-7-11(32)13(34)19(45-7)30-5-26-9-16(30)27-20(22)28-17(9)35;;;/h3-7,10-13,18-19,31-34H,1-2H2,(H,36,37)(H,38,39)(H,40,41)(H2,21,23,24)(H3,22,27,28,35);3*1H3/t6-,7-,10?,11?,12+,13+,18-,19-;;;/m1.../s1 |
| InChIKey | JKQIMADCKODNKE-JYSBNCGYSA-N |
| SMILES | C1=NC(=C2C(=N1)N(C=N2)C3C(C(C(O3)COP(=O)(O)OP(=O)(O)OP(=O)(O)OCC4C(C(C(O4)N5C=NC6=C5N=C(NC6=O)N)O)O)O)O)N.N.N.N |
| Reference | [1]. Renata Kasprzyk, et al. Direct High-Throughput Screening Assay for mRNA Cap Guanine-N7 Methyltransferase Activity. Chemistry. 2020 Sep 1;26(49):11266-11275. [2]. Dennis Reichert, et al. Light-control of cap methylation and mRNA translation via genetic code expansion of Ecm1. Chem Sci. 2021 Feb 8;12(12):4383-4388. |
