GW-6604

• CAT Number: I007153
• CAS Number: 452342-37-9
• Molecular Formula: C19H14N4
• Molecular Weight: 298.34
• Purity: ≥95%
• Synonyms: 2-phenyl-4-(5-pyridin-2-yl-1H-pyrazol-4-yl)pyridine
• InChI: InChI=1S/C19H14N4/c1-2-6-14(7-3-1)18-12-15(9-11-21-18)16-13-22-23-19(16)17-8-4-5-10-20-17/h1-13H,(H,22,23)
• InChIKey: BDCBRQYHYNUWAM-UHFFFAOYSA-N
• SMILES: C1=CC=C(C=C1)C2=NC=CC(=C2)C3=C(NN=C3)C4=CC=CC=N4

GW-6604 is an ALK5 inhibitor with an IC50 value of 140 nM for inhibiting its autophosphorylation, and can be used in the study of liver fibrosis[1].
GW-6604 (0-10 μM, ) inhibits TGF-β-induced PAI-1 transcription and secretion in HepG2 cells with an IC50 value of 500 nM, effectively blocking the Smad-dependent response[1].
GW-6604(p.o., 25-80 mg/kg, twice a day, 3 weeks) dependently inhibits hepatic COL IA1 overexpression in an acute DMN model in SD rats, reducing its expression by 80%. Normal liver weight was maintained, liver fibrosis was effectively reduced and matrix degeneration was increased[1].

Reference

[1]. Anne-Charlotte de Gouville, et al. Inhibition of TGF-beta signaling by an ALK5 inhibitor protects rats from dimethylnitrosamine-induced liver fibrosis. Br J Pharmacol. 2005 May;145(2):166-77. 
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