H-Ala-Pro-AFC

• CAT Number: M108477
• CAS Number: 125791-92-6
• Molecular Formula: C18H18F3N3O4
• Molecular Weight: 397.35
• Purity: ≥95%
• Synonyms: (2S)-1-[(2S)-2-aminopropanoyl]-N-[2-oxo-4-(trifluoromethyl)chromen-7-yl]pyrrolidine-2-carboxamide
• InChI: InChI=1S/C18H18F3N3O4/c1-9(22)17(27)24-6-2-3-13(24)16(26)23-10-4-5-11-12(18(19,20)21)8-15(25)28-14(11)7-10/h4-5,7-9,13H,2-3,6,22H2,1H3,(H,23,26)/t9-,13-/m0/s1
• InChIKey: GGRRKBLWOMKWNS-ZANVPECISA-N
• SMILES: CC(C(=O)N1CCCC1C(=O)NC2=CC3=C(C=C2)C(=CC(=O)O3)C(F)(F)F)N

H-Ala-Pro-AFC(CAT: M108477) is a fluorogenic tripeptide substrate composed of alanine and proline linked to 7-amino-4-trifluoromethylcoumarin (AFC). This compound is widely used in enzymatic assays to measure protease activity, particularly for proline-specific proteases and other peptidases. Upon enzymatic cleavage, the non-fluorescent peptide releases highly fluorescent AFC, enabling sensitive detection and quantification. Its structure makes it ideal for high-throughput screening and kinetic studies in biochemical and pharmaceutical research. H-Ala-Pro-AFC is a valuable tool for investigating enzyme specificity, inhibitor potency, and real-time enzymatic reactions in fields such as cancer, inflammation, and metabolic disease research.