For research use only. Not for therapeutic Use.
HBC599 is a HBC analog. HBC is nonfluorescent in solution, but emits strong fluorescence upon forming tight complex with Pepper RNA aptamer. HBC-Pepper complex can be used to visualize RNA dynamics in live cells[1].
Pepper can activate the fluorescence of HBC. Mammalian cells expressing Pepper exhibits robust greenyellowish fluorescence on incubation with 1 μM HBC[1].
Peppers allow simple and robust imaging of diverse RNA species in live cells with minimal perturbation of the target RNA’s transcription, localization and translation[1].
| Catalog Number | I045894 |
| CAS Number | 2530162-00-4 |
| Synonyms | 4-[(Z)-1-cyano-2-[6-[2-hydroxyethyl(methyl)amino]-1-benzothiophen-2-yl]ethenyl]benzonitrile |
| Molecular Formula | C21H17N3OS |
| Purity | ≥95% |
| InChI | InChI=1S/C21H17N3OS/c1-24(8-9-25)19-7-6-17-10-20(26-21(17)12-19)11-18(14-23)16-4-2-15(13-22)3-5-16/h2-7,10-12,25H,8-9H2,1H3/b18-11+ |
| InChIKey | WXTHEUULEXARCU-WOJGMQOQSA-N |
| SMILES | CN(CCO)C1=CC2=C(C=C1)C=C(S2)C=C(C#N)C3=CC=C(C=C3)C#N |
| Reference | [1]. Chen X, et al. Visualizing RNA dynamics in live cells with bright and stable fluorescent RNAs. Nat Biotechnol. 2019 Nov;37(11):1287-1293. |
