For research use only. Not for therapeutic Use.
Thioester analogs of glycerophospholipids, in combination with Ellman’s reagent, are convenient colorimetric substrates for the measurement of phospholipase (PL) activity. Diheptanoyl thio-<wbr></wbr>PC is a commonly-<wbr></wbr>used colorimetric substrate for all PLA<sub>2</sub>s, with the exception of cPLA<sub>2</sub> and platelet-<wbr></wbr>activating factor acetylhydrolase (PAF-<wbr></wbr>AH). Heptanoyl thio-<wbr></wbr>PC is an analog of diheptanoyl thio-<wbr></wbr>PC that contains an ether-<wbr></wbr>linked saturated C<sub>16</sub> moiety at the <em>sn</em>-<wbr></wbr>1 position rather than a heptanoyl thiol ester. Porcine pancreatic and bee venom sPLA<sub>2</sub> enzymes exhibit 10-<wbr></wbr>13 fold less activity when assayed with heptanoyl thio-<wbr></wbr>PC compared to diheptanoyl thio-<wbr></wbr>PC. This decrease in activity has not been thoroughly investigated.
| Catalog Number | R011230 |
| Synonyms | 1-O-hexadecyl-2-deoxy-2-thio-R-(heptanoyl)-sn-glyceryl-3-phosphocholine |
| Molecular Formula | C31H64NO6PS |
| Purity | ≥95% |
| Storage | -20°C |
| InChI | InChI=1S/C31H64NO6PS/c1-6-8-10-12-13-14-15-16-17-18-19-20-21-23-26-36-28-30(40-31(33)24-22-11-9-7-2)29-38-39(34,35)37-27-25-32(3,4)5/h30H,6-29H2,1-5H3/t30-/m1/s1 |
| InChIKey | HWBAKTCCNWQOTL-SSEXGKCCSA-N |
| SMILES | CCCCCCCCCCCCCCCCOC[C@H](COP(=O)(O)OCC[N](C)(C)C)SC(=O)CCCCCC |
| Reference | 1.Hendrickson, H.S.,Hendrickson, E.K., and Dybvig, R.H. Chiral synthesis of a dithiolester analog of phosphatidylcholine as a substrate for the assay of phospholipase A2. Journal of Lipid Research 24, 1532-1537 (1983). |
