For research use only. Not for therapeutic Use.
HKOCl-4 (BXY2142) is a rhodol-based yellow fluorescent probe for the detection of hypochlorous acid with excellent sensitivity and selectivity[1]. HKOCl-4 has longer absorption wavelength and better pH stability compared with fluorescein-based probes. Ex: 530 nm; Em 557 nm.
HKOCl-4 (10 μM) shows an increasing florescence intensity with increasing concentration of hypochlorous acid[1].
HKOCl-4 exhibits a ultra-fast response towards 1 equiv of HOCl (reached a plateau within 2 min) , and it shows a >20-fold increase in fluorescence intensity over other analytes (1 equiv of OH and ONOO as well as 10 equiv of O2, NO, 1O2, ROO , TBHP and H2O2)[2].
HKOCl-4 is first tested and it was proven to be non-toxic in living cells even at a concentration up to 50 μM in RAW264.7 macrophages[2].
| Catalog Number | I045481 |
| CAS Number | 2031170-85-9 |
| Synonyms | ethyl 4-[9′-(3,5-dichloro-4-hydroxyphenoxy)-2′,2′,4′-trimethyl-3-oxospiro[2-benzofuran-1,6′-3,4-dihydrochromeno[3,2-g]quinoline]-1′-yl]butanoate |
| Molecular Formula | C38H35Cl2NO7 |
| Purity | ≥95% |
| InChI | InChI=1S/C38H35Cl2NO7/c1-5-45-34(42)11-8-14-41-31-19-33-28(18-25(31)21(2)20-37(41,3)4)38(26-10-7-6-9-24(26)36(44)48-38)27-13-12-22(17-32(27)47-33)46-23-15-29(39)35(43)30(40)16-23/h6-7,9-10,12-13,15-19,21,43H,5,8,11,14,20H2,1-4H3 |
| InChIKey | LUSCIMHITFMNOM-UHFFFAOYSA-N |
| SMILES | CCOC(=O)CCCN1C2=CC3=C(C=C2C(CC1(C)C)C)C4(C5=C(O3)C=C(C=C5)OC6=CC(=C(C(=C6)Cl)O)Cl)C7=CC=CC=C7C(=O)O4 |
| Reference | [1]. Dan Yang, et al. Diarylether-based fluorogenic probes for detection of hypochlorous acid or hydroxyl radical. Patent US20160312033. [2]. Xiaoyu Bai, et al. HKOCl-4: a rhodol-based yellow fluorescent probe for the detection of hypochlorous acid in living cells and tissues. |
