HKOCl-4m

• CAT Number: I045006
• CAS Number: 2031170-88-2
• Molecular Formula: C63H61BrCl2N3O7P
• Molecular Weight: 1153.96
• Purity: ≥95%
• Synonyms: [5-[4-[4-[9′-(3,5-dichloro-4-hydroxyphenoxy)-2′,2′,4′-trimethyl-3-oxospiro[2-benzofuran-1,6′-3,4-dihydrochromeno[3,2-g]quinoline]-1′-yl]butanoyl]piperazin-1-yl]-5-oxopentyl]-triphenylphosphanium;bromide
• InChI: InChI=1S/C63H60Cl2N3O7P.BrH/c1-42-41-62(2,3)68(30-17-27-59(70)67-33-31-66(32-34-67)58(69)26-15-16-35-76(45-18-7-4-8-19-45,46-20-9-5-10-21-46)47-22-11-6-12-23-47)55-40-57-52(39-49(42)55)63(50-25-14-13-24-48(50)61(72)75-63)51-29-28-43(38-56(51)74-57)73-44-36-53(64)60(71)54(65)37-44;/h4-14,18-25,28-29,36-40,42H,15-17,26-27,30-35,41H2,1-3H3;1H
• InChIKey: ZLHCHNQSAGGVMV-UHFFFAOYSA-N
• SMILES: CC1CC(N(C2=CC3=C(C=C12)C4(C5=C(O3)C=C(C=C5)OC6=CC(=C(C(=C6)Cl)O)Cl)C7=CC=CC=C7C(=O)O4)CCCC(=O)N8CCN(CC8)C(=O)CCCC[P+](C9=CC=CC=C9)(C1=CC=CC=C1)C1=CC=CC=C1)(C)C.[Br-]

HKOCl-4m is a selective and mitochondria-targeting rhodol-based fluorescent probe for monitoring mitochondrial hypochlorous acid (HOCl)[1].
HKOCl-4m (5 μM; 30 min) exhibits robust increase in fluorescence intensity upon addition of HOCl. HKOCl-4m has been successfully applied in detecting endogenous HOCl in RAW264.7 mouse macrophages. HKOCl-4m could be applied for monitoring the mitochondrial HOCl formation in living cells[1].

Reference

[1]. Xiaoyu Bai, et al. HKOCl-4: a rhodol-based yellow fluorescent probe for the detection of hypochlorous acid in living cells and tissues. Org. Chem. Front, 2020.