HKSOX-1r (5/6-mixture)

• CAT Number: I045484
• Molecular Formula: C29H15F10NO14S2
• Molecular Weight: 855.54
• Purity: ≥95%
• Synonyms: methyl 2-[(2-methoxy-2-oxoethyl)-[2′,4′,5′,7′-tetrafluoro-1-oxo-3′,6′-bis(trifluoromethylsulfonyloxy)spiro[2-benzofuran-3,9′-xanthene]-5-carbonyl]amino]acetate;methyl 2-[(2-methoxy-2-oxoethyl)-[2′,4′,5′,7′-tetrafluoro-3-oxo-3′,6′-bis(trifluoromethylsulfonyloxy)spiro[2-benzofuran-1,9′-xanthene]-5-carbonyl]amino]acetate
• InChI: InChI=1S/2C29H15F10NO14S2/c1-49-17(41)8-40(9-18(42)50-2)25(43)10-3-4-12-11(5-10)26(44)52-27(12)13-6-15(30)23(53-55(45,46)28(34,35)36)19(32)21(13)51-22-14(27)7-16(31)24(20(22)33)54-56(47,48)29(37,38)39;1-49-17(41)8-40(9-18(42)50-2)25(43)10-3-4-11-12(5-10)27(52-26(11)44)13-6-15(30)23(53-55(45,46)28(34,35)36)19(32)21(13)51-22-14(27)7-16(31)24(20(22)33)54-56(47,48)29(37,38)39/h2*3-7H,8-9H2,1-2H3
• InChIKey: OFMLXQFBPXOWPN-UHFFFAOYSA-N
• SMILES: COC(=O)CN(CC(=O)OC)C(=O)C1=CC2=C(C=C1)C3(C4=CC(=C(C(=C4OC5=C(C(=C(C=C53)F)OS(=O)(=O)C(F)(F)F)F)F)OS(=O)(=O)C(F)(F)F)F)OC2=O.COC(=O)CN(CC(=O)OC)C(=O)C1=CC2=C(C=C1)C(=O)OC23C4=CC(=C(C(=C4OC5=C(C(=C(C=C35)F)OS(=O)(=O)C(F)(F)F)F)F)OS(=O)(=O)C(F)(F)F)F

HKSOX-1r (5/6-mixture) is a fluorescent probe which is used for imaging and detection of endogenous superoxide in live cells and in vivo. HKSOX-1r (5/6-mixture) exhibits excellent selectivity and sensitivity towards superoxide anion radical[1].
HKSOX-1r (5/6-mixture) (2 μM; 30 min) detects mitochondrial respiratory inhibitor-induced O2?- formation in a highly sensitive and rapid manner in HCT116, BV-2 and RAW264.7 cells[1].
HKSOX-1r (5/6-mixture) (10 μM; 20 min) detects distinct fluorescence distribution in zebrafish embryos are subjected to challenge of PMA or Antimycin A[1].

Reference

[1]. Hu JJ, et, al. Fluorescent Probe HKSOX-1 for Imaging and Detection of Endogenous Superoxide in Live Cells and In Vivo. J Am Chem Soc. 2015 Jun 3;137(21):6837-43.
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