Hoechst 33342

• CAT Number: I002780
• CAS Number: 23491-52-3
• Molecular Formula: C27H28N6O
• Molecular Weight: 452.55
• Purity: ≥95%
• Synonyms: 2′-(4-ethoxyphenyl)-6-(4-methylpiperazin-1-yl)-1H,3’H-2,5′-bibenzo[d]imidazole
• Target: Autophagy
• Solubility: DMSO or water Protect from light
• Storage: Store at -20°C
• IUPAC Name: 2-(4-ethoxyphenyl)-6-[6-(4-methylpiperazin-1-yl)-1H-benzimidazol-2-yl]-1H-benzimidazole
• InChI: InChI=1S/C27H28N6O.3ClH/c1-3-34-21-8-4-18(5-9-21)26-28-22-10-6-19(16-24(22)30-26)27-29-23-11-7-20(17-25(23)31-27)33-14-12-32(2)13-15-33;;;/h4-11,16-17H,3,12-15H2,1-2H3,(H,28,30)(H,29,31);3*1H
• InChIKey: JABNPSKWVNCGMX-UHFFFAOYSA-N
• SMILES: CN1CCN(C2=CC=C3C(N=C(C4=CC=C5C(N=C(C6=CC=C(OCC)C=C6)N5)=C4)N3)=C2)CC1.[H]Cl.[H]Cl.[H]Cl

Hoechst 33342 (CAT: I002780) is a fluorescent dye and a cell-permeable DNA stain commonly used in molecular biology and cell biology research. It binds to the minor groove of double-stranded DNA, exhibiting preferential binding to AT-rich regions. Hoechst 33342 is widely used for nuclear staining and DNA content analysis in live or fixed cells. It is also employed in fluorescence microscopy and flow cytometry to visualize and quantify DNA, identify cell cycle phases, and assess cell viability. Furthermore, Hoechst 33342 has been utilized in studies involving DNA damage, apoptosis, and cell sorting based on DNA content.